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RESEARCH ARTICLE
Contents Vol 31(8)

Effects of UV irradiation on barley and tomato leaves: thermoluminescence as a method to screen the impact of UV radiation on crop plants

Matthias Gilbert A B , Jiri Skotnica A , Ilka Weingart A and Christian Wilhelm A
+ Author Affiliations
- Author Affiliations

A University of Leipzig, Institute of Botany, Plant Physiology, Johannisallee 21-23, D-04103 Leipzig, Germany.

B Corresponding author; email: mgilbert@rz.uni-leipzig.de

Functional Plant Biology 31(8) 825-845 https://doi.org/10.1071/FP03186
Submitted: 14 October 2003  Accepted: 26 April 2004   Published: 23 August 2004

Abstract

The effect of different UV intensities and irradiation times on barley and tomato leaves was investigated by analysis of thermoluminescence (TL) and chlorophyll (chl) fluorescence measurements. Epifluorescence microscopy was used to estimate the epidermal UV transmittance of leaves. In barley a strong supression of TL emission from the S2QB– (B-band) and the S2QA– (Q-band) charge recombination was observed increasing with prolonged UV exposure. Primary barley leaves were more sensitive to UV than secondary leaves. In tomato plants a decrease in the B-band only takes place at very high UV intensities and after prolonged exposure times (4 h). The impact of UV in cotyledons was more pronounced than in pinnate leaves of tomato plants. The strong differences in sensitivity to UV in the investigated barley and tomato variety may be due to different concentrations of UV screening pigments in the epidermal layer as demonstrated by epifluorescence measurements. The results show that TL has the same potential to analyse the sensitivity or tolerance of crop plants to UV irradiation as routine fluorescence techniques. Furthermore, TL is directly monitoring the radical pair states of PSII and can distinguish between UV-induced donor and acceptor site-related damage.

Keywords: epidermal UV transmittance, Hordeum, Lycopersicon, PSII, stress tolerance, thermoluminescence, UV, variable fluorescence, water-splitting apparatus.


Acknowledgments

This research has been supported by the German Ministery of Education and Research (BMBF), grant no. 0339876. Seeds of the tomato variety Yellow Cherry were supplied by the Institute of Plant Genetics and Crop Plant Research (IPK) in Gatersleben, Germany. Seeds of the barley variety were a kind gift of Professor Dr B. Grimm (Humboldt University, Berlin).


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