Organ-Specific Expression of Three Peroxidase-Encoding Cdnas From Lucerne (Medicago sativa)

Abstract

Three putative peroxidase-encoding cDNA clones have been isolated from lucerne (Medicago sativa) using an oligonucleotide probe based on the conserved FHDCFV amino acid sequence domain of peroxidase proteins. The pxdA, pxdC and pxdD genes are specifically expressed in leaf, root or stem tissue, respectively. The stem-specific gene, pxdD, is shown by tissue print hybridisation analysis to be expressed in the phloem and collenchyma cells of lucerne tissue.