Free Standard AU & NZ Shipping For All Book Orders Over $80!
Register      Login
Functional Plant Biology Functional Plant Biology Society
Plant function and evolutionary biology
RESEARCH ARTICLE

Molecular cloning and expression of a cDNA encoding a microsomal w-6 fatty acid desaturase from cotton (Gossypium hirsutum)

Qing Liu, Surinder P. Singh, Curt L. Brubaker, Peter J. Sharp, Allan G. Green and D. R. Marshall

Australian Journal of Plant Physiology 26(2) 101 - 106
Published: 1999

Abstract

A cDNA (ghFAD2-1) encoding a seed-specific microsomal ω-6 desaturase was isolated from a cotton (Gossypium hirsutum L. cv. Deltapine-16) embryo cDNA library. The deduced amino acid sequence showed substantial similarity to other plant microsomal ω-6 desaturases. Northern blot analysis indicated that the ghFAD2-1 transcript was specifically induced during embryo development and expression of the transcript could not be detected in leaves. Southern blot analysis using the coding region and 3′ untranslated region of ghFAD2-1 revealed that microsomal ω-6 desaturase in cotton is encoded by a small multigene family. There are at least two copies of ghFAD2-1 in two tetraploid cotton species (G. hirsutum L. and G. barbadense L.) and at least one copy in diploid cotton species (G. herbaceum L., G. raimondii Ulbrich and G. robinsonii Mueller).

Keywords: cottonseed, Gossypium hirsutum, fatty acid, ω -6 desaturase.

https://doi.org/10.1071/PP98118

© CSIRO 1999

Committee on Publication Ethics


Export Citation Get Permission

View Dimensions

View Altmetrics