Carbon isotope discrimination in leaf and stem sugars, water-use efficiency and mesophyll conductance during different developmental stages in rice subjected to drought

Abstract

Carbon isotope discrimination (Δ), growth analysis, water-use efficiency (WUE) and gas exchange characteristics were studied in rice plants (Oryza sativa L.) subjected to drought during different developmental stages. Drought caused major effects on growth, WUE, Δ and photosynthetic CO₂ assimilation. Substantial differences in the Δ of the bulk biomass among different organs and in carbohydrates extracted from leaves and stems were observed. Possible influences of chemical composition, fractionation during translocation and seasonal changes in the ratio of intercellular and atmospheric partial pressures of CO₂ on such differences in Δ are discussed. Stem carbohydrate Δ was correlated with relative growth rate, and, during early grain filling, was negatively correlated with WUE measured between flowering and early grain filling. Δ in leaf sugars was used to estimate mesophyll conductance (g_m), the conductance to CO₂ diffusion inside leaves, from the intercellular air spaces to the chloroplast. During ontogeny, g_m showed a marked progressive decrease, evident in both droughted plants and fully irrigated controls. There was a positive correlation between the rate of CO₂ assimilation and g_m. The analysis of Δ in leaf and stem carbohydrates is proposed as a useful indicator of growth, WUE and photosynthetic parameters relevant for yield of rice under drought-prone conditions.