Arsenic Uptake by Barley Seedlings

Abstract

Arsenate uptake consists of a rapid, apparently irreversible, initial phase followed by a less rapid 'steady state' phase, both of which are temperature sensitive and strongly inhibited by phosphate. By comparison, 'steady state' uptake of phosphate was only weakly inhibited by arsenate. The initial and 'steady state' phases of arsenate uptake were highly correlated (R = 0.958, P < 0.001). Arsenite also appeared to exhibit initial and 'steady state' uptake phases but uptake was slower by a factor of 3-4 than for arsenate at the same concentration. The results are discussed in relation to recent work on a DNP-sensitive initial binding step in the uptake of other elements.