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Functional Plant Biology Functional Plant Biology Society
Plant function and evolutionary biology
RESEARCH ARTICLE

Comparison of three selectable marker genes for transformation of wheat by microprojectile bombardment

Barbara Witrzens, Richard I.S. Brettell, Fiona R. Murray, David McElroy, Zhongyi Li and Elizabeth S. Dennis

Australian Journal of Plant Physiology 25(1) 39 - 44
Published: 1998

Abstract

Three selectable marker genes were compared for their efficacy in the production of transgenic wheat plants following microprojectile bombardment of cultured immature embryos. While transformed plants were recovered using the bar (phosphinothricin acetyltransferase) gene in combination with bialaphos, and the aphA (neomycin phosphotransferase) gene in combination with geneticin or paromomycin, no transgenic material was obtained with the hpt (hygromycin phosphotransferase) gene and hygromycin B. Southern analysis revealed single copy as well as multiple copy insertions of the bar and aphA transgenes. Inheritance of these selectable marker genes was demonstrated in the T1 generation progenies.

Keywords: transformation, wheat, biolistics, selectable markers, antibiotic, herbicide.

https://doi.org/10.1071/PP97095

© CSIRO 1998

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