
TY  - JOUR
A1  - Chapple, RM 
A1  - Chaudhury, AM 
A1  - Blomer, KC 
A1  - Farrell, LB 
A1  - Dennis, ES 
T1  - Construction of a YAC Contig of 2 Megabases Around the MS1 Gene in <I>Arabidopsis thaliana</I>
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 1996
VL  - 23
IS  - 4
SP  - 453
EP  - 465
KW  - 
UR  - https://doi.org/10.1071/PP9960453 
N2  - The ms1 mutation of Arabidopsis thaliana causes male sterility by preventing the development of normal microspores in the developing anther. The gene is located on a region of chromosome 5 containing the RFLP markers g4111, g4560 and g21503. Using yeast artificial chromosome (YAC) libraries, we have constructed a contig of 38 YACs spanning approximately 2.1 megabases (approximately 2% of the genome) around MS1 and redefined the order of these RFLP markers. Chimeric YACs and repetitive DNA caused problems in chromosome 'walking'. A method for cloning YAC right ends by plasmid rescue was applied to arabidopsis. One YAC end contained a portion of the A. thaliana sucrose synthase gene ASUSI, hence locating this gene on chromosome 5 near MS1. Using recombinant plant populations containing ms1 and flanking markers, MS1 was localised to a 200 kb region within the YAC contig. In this contig the relationship between physical and genetic distance varied from less than 100 kb to 720 kb per centimorgan. 
ER  -

