
TY  - JOUR
A1  - Underhill, SJR 
A1  - Critchley, C 
T1  - Cellular Localisation of Polyphenol Oxidase and Peroxidase Activity in <I>Litchi chinensis</I> Sonn. Pericarp
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 1995
VL  - 22
IS  - 4
SP  - 627
EP  - 632
KW  - 
UR  - https://doi.org/10.1071/PP9950627 
N2  - Cellular localisation of visual browning and oxidative activity studies were conducted to determine the relative significance of polyphenol oxidase (PPO) and peroxidase (POD) activities during pericarp browning. Pericarp browning was first observed on the protuberance apices and subsequently extended uniformly over the entire pericarp surface. Anatomically, browning was highly localised and restricted to the epicarp and the upper mesocarp. PPO and POD activities were highest in the epicarp, with progressively less activity in both the mesocarp and endocarp. In situ localisation of oxidative activity using tissue blots confirmed high epicarp PPO activity. POD activity, although primarily restricted to mesocarp vascular tissue, was also detected in the epicarp. We believe that litchi pericarp browning is due to highly localised oxidative activity in the epicarp and upper mesocarp. As PPO and POD activities were significantly higher in this tissue and browning was not observed when both enzymes were selectively inhibited, it is postulated that both PPO and POD activities are associated with litchi pericarp browning. The current theory that litchi pericarp browning is only caused by PPO activity needs to be re-appraised to determine the relative role of POD activity. 
ER  -

