
TY  - JOUR
A1  - Mcintyre, CL 
A1  - Rae, AL 
A1  - Curtis, MD 
A1  - Manners, JM 
T1  - Sequence and Expression of a Caffeic Acid O-Methyl Transferase Cdna Homologue in the Tropical Forage Legume Stylosanthes Humilis.
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 1995
VL  - 22
IS  - 3
SP  - 471
EP  - 478
KW  - 
UR  - https://doi.org/10.1071/PP9950471 
N2  - The isolation and characterisation of a cDNA encoding a caffeic acid 0-methyl transferase cDNA homologue (COMT) from Stylosanthes humilis are described. The clone is 1391 nucleotides in length, with an open reading frame encoding a predicted protein of 366 amino acids. Cluster analysis of the deduced amino acid sequence revealed extensive homology to other published O-methyl transferase sequences. Maximum levels of homology were seen with COMTs from alfalfa (87%) and aspen (84%). Southern analysis suggested that this enzyme is encoded by two genes in S. humilis. The mRNA is most strongly expressed in stem tissue, with intermediate levels of expression in young leaves and roots, and does not appear to be induced upon fungal infection or wounding. 
ER  -

