
%0 Journal Article
%A Rathjen, H 
%A Robinson, SP 
%T Characterisation of a Variegated Grapevine Mutant Showing Reduced Polyphenol Oxidase Activity
%J Functional Plant Biology
%D 1992/02/01
%V 19
%N 1
%P 43-54
%K 
%R https://doi.org/10.1071/PP9920043
%U https://www.publish.csiro.au/paper/PP9920043 
%X Fruit of the variegated grapevine mutant Bruce's Sport is known to dry to a lighter colour than other seedless varieties. The biochemical basis for this decreased browning capacity was investigated. Bruce's Sport grapes had similar levels of phenolic compounds to Sultana H5. Activity of the enzyme polyphenol oxidase (PPO EC 1.10.3.1) in mature berries of Bruce's Sport was only 20-30% of that in Sultana H5. No evidence of inhibitors or activators of PPO was found when berry extracts were mixed. PPO activity on a fresh weight basis was highest in the grape seed traces, intermediate in the skin, and lowest in the pulp in both varieties. In each tissue type, however, PPO activity in Bruce's Sport was less than 25% of that in Sultana H5. On a fresh weight basis, PPO activity in Sultana H5 berries was high at fruit set then declined as the berry developed. PPO activity per berry increased from fruit set until veraison, then remained constant. PPO activity showed similar changes during development of Bruce's Sport berries but was lower than in Sultana H5 at all stages. The Bruce's Sport grapes were variegated and the green regions of skin had similar PPO activity to Sultana H5 skin while the white regions had very low activity. Only the green regions of skin of Bruce's Sport grapes stained for PPO activity with endogenous or exogenously applied phenolic substrates. The decreased browning in this grapevine mutant apparently results from decreased levels of PPO activity in the white regions of the berry, possibly arising from a disruption in chloroplast development. 
