
TY  - JOUR
A1  - Babourina, Olga 
A1  - Shabala, Sergey 
A1  - Newman, Ian 
T1  - Verapamil-induced kinetics of ion flux in oat seedlings
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 2000
VL  - 27
IS  - 11
SP  - 1031
EP  - 1040
KW  - Avena sativa L., Ca 2+ , Cl &#8211; , H + , K + , Na + , verapamil.
UR  - https://doi.org/10.1071/PP99182 
N2  - Verapamil application in ion transport studies on plant cells is widespread; however, the mechanism of its action is still poorly understood. Net flux of Ca 2+ , K + , Na + , H + and Cl &#8211; were measured in solution around oat seedlings using the non-invasive ion-selective microelectrode MIFE technique. The verapamil effect on intact plant tissues was a distinct immediate influx of monovalent cations, H + , K + or Na + . Pre-treatment with tetraethyl-ammonium, Cs + , Ba 2+ or Ca 2+ did not affect K + flux changes, whereas DMSO, Cd 2+ and vanadate increased verapamil-induced K + influx. Verapamil-induced K + flux increased with increased external KCl concentration or pH. Verapamil concentration up to 1 mM failed to shift Ca 2+ flux to efflux. Pre-treatment with adrenaline and dopamine agonists and antagonists led to changes in verapamil-induced ion flux, especially for Ca 2+ . We suggest that a transporter that allows entry of K + and Na + was the main system providing monovalent ion influx after verapamil application. 
ER  -

