
TY  - JOUR
A1  - Rosiak-Figielek, Beata 
A1  - Jackowski, Grzegorz 
T1  - The disappearance kinetics of Lhcb polypeptides during dark-induced senescence of leaves
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 2000
VL  - 27
IS  - 3
SP  - 245
EP  - 251
KW  - antibodies, barley, degradation, leaf, Lhcb, polypeptide, senescence.
UR  - https://doi.org/10.1071/PP99040 
N2  - A set of polyclonal antibodies in conjunction with immunoblot technique and integrating densitometry has been used to positively identify and quantify Lhcb 1-6 polypeptides in barley (Hordeum vulgare L.) leaves in which senescence processes were induced by detachment and dark incubation for 0&#8211;6 d. A considerable hetero-geneity with regard to disappearance kinetics on a chlorophyll basis during the course of senescence has been foundamong individual Lhcbs. Lhcb 2/1 (the heavier of two polypeptides representing barley Lhcb2) and Lhcb3 exhibited the most rapid disappearance kinetics and decreased to 20 and 42% of their initial levels, respectively, after 6 d of dark incubation. Lhcb 1, 4 and 6 levels were maintained through the senescence, at a level very similar to that of fresh leaves, while Lhcb 2/2 (the lighter of two polypeptides representing barley Lhcb 2) and Lhcb 5 were the most stable of all Lhcbs &#8212; their relative abundance increased after 6 d of dark incubation of the leaves to 181 and 120%, respectively, of the value detected in fresh leaves. 
ER  -

