
TY  - JOUR
A1  - Millhouse, J 
A1  - Wiskich, JT 
A1  - Beevers, H 
T1  - Metabolite Oxidation and Transport in Mitochondria of Endosperm From Germinating Castor Bean
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 1983
VL  - 10
IS  - 2
SP  - 167
EP  - 177
KW  - 
UR  - https://doi.org/10.1071/PP9830167 
N2  - Mitochondria from germinating castor bean endosperm were purified on linear sucrose-density gradients. It is shown that succinate and malate plus glutamate are oxidized rapidly; other substrates including malate (via malic enzyme) and external NADH are oxidized slowly. It is proposed that the oxidation of NADH produced during &#946;-oxidation and the glyoxylate cycle occurs intramitochondrially via malate. Castor bean mitochondria are relatively impermeable to oxaloacetate and a malate-aspartate type shuttle is required. Metabolite transport and exchange properties support the operation of malatein (or succinatein)/2-oxoglutarateout and glutamatein/aspartateout shuttles. Not all the exchange systems were reversible. The results support proposed schemes for metabolite transfer between glyoxysomes and mitochondria of castor bean endosperm during germination. 
ER  -

