
%0 Journal Article
%A Donovan, GR 
%A Lee, JW 
%A Longhurst, TJ 
%T Cell-Free Synthesis of Wheat Prolamins
%J Functional Plant Biology
%D 1982/02/01
%V 9
%N 1
%P 59-68
%K 
%R https://doi.org/10.1071/PP9820059
%U https://www.publish.csiro.au/paper/PP9820059 
%X Total RNA, polyribosomes and polyribosomal RNA have been isolated from immature wheat grain and used to prime the wheat-germ cell-free protein synthesis system to produce storage protein. The synthesis of wheat prolamins, a major fraction of storage proteins, was mainly associated with membrane-bound polyribosomes. The ratio of bound to free polyribosomes increased from one at 8 days to approximately three at 32 days post-anthesis. SDS polyacrylamide gel electrophoresis and fluorography of the prolamins showed that the same components were being synthesized and accumulated from 12 days post-anthesis onward. Evidence is presented that prolamins within the molecular weight range 35 000-45 000 may be translated with a signal sequence attached. These signal sequences are rapidly removed during translation and further post-translational modifications may occur over longer periods. 
