
TY  - JOUR
A1  - Mascara, T 
A1  - Fincher, GB 
T1  - Biosynthesis of Arabinogalactan-Protein in <I>Lolium multiflorum</I> (Ryegrass) Endosperm Cells. II. <I>In vitro</I> Incorporation of Galactosyl Residues From UDPgalactose Into Polymeric Products
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 1982
VL  - 9
IS  - 1
SP  - 31
EP  - 45
KW  - 
UR  - https://doi.org/10.1071/PP9820031 
N2  - When mixed-membrane fractions from suspension-cultured Lolium multiflorum endosperm cells are incubated in vitro with UDP-[14C]galactose, 66% ethanol-insoluble products of apparent molecular weight greater than 60 000 are labelled in both galactosyl and glucosyl residues, suggesting that an active UDPgalactose 4-epimerase is present on the membrane fraction. The epimerase can be inhibited with ADPribose, to produce polymeric material in which [14C]galactosyl residues pre-dominate. While some of these residues appear to be associated with glycoproteins, affinity chromatography of the products on mouse myeloma protein J539-Sepharose provides evidence that &#946;-galactans containing 1,6-linkages are amongst the products.    Monosaccharide analyses and methylation data indicate that the mixed-membrane preparations contain associated polysaccharide of structure analogous to the 1,3;1,4-&#946;-glucans, arabinoxylans and arabino-3,6-galactans normally found in cell walls or secreted into the medium. 
ER  -

