
TY  - JOUR
A1  - Gifford, RM 
A1  - Bremner, PM 
T1  - Accumulation and Conversion of Sugars by Developing Wheat Grains. II. Light Requirement for Kernels Cultured <I>in vitro</I>
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 1981
VL  - 8
IS  - 6
SP  - 631
EP  - 640
KW  - 
UR  - https://doi.org/10.1071/PP9810631 
N2  - Light stimulated the incorporation of [14C]sucrose into the starch of developing wheat kernels cultured in liquid medium. The light response curve saturated at about one-tenth full sunlight. Surgical removal of the green-layer of the inner pericarp, or inhibition of its photosynthesis by 3-(3,4-dichlorophenyl)-1,1-dimethylurea, eliminated the light response. Experiments with different gases above the culture medium suggested that photosynthetic generation of oxygen by the green- layer was a possible cause of the response.    Accumulation of label from [14C]sucrose into the alcohol-soluble fraction of the grain was slightly light-sensitive. This effect also saturated at about one-tenth full sunlight but there was no evidence that it was related to the photosynthetic behaviour of the green-layer.    These observations are discussed in relation to in vitro culturing methodology, to the layered structure of wheat starch granules, and to the role of the green-layer of the inner pericarp of the wheat kernel. 
ER  -

