
%0 Journal Article
%A Richardson, Kim 
%A Fowler, Sarah 
%A Pullen, Carly 
%A Skelton, Caryl 
%A Morris, Bret 
%A Putterill, Jo 
%T T-DNA tagging of a flowering-time gene and improved gene transfer by <emph type="2">in planta</emph> transformation of <emph type="2">Arabidopsis</emph>
%J Functional Plant Biology
%D 1998/03/30
%V 25
%N 1
%P 125-130
%K Arabidopsis, T-DNA tagging, in planta transformation, flowering-time gene, GIGANTEA.
%R https://doi.org/10.1071/PP97109
%U https://www.publish.csiro.au/paper/PP97109 
%X Gene tagging with insertional mutagens greatly facilitates the isolation of novel genes. A new collection of Arabidopsis T-DNA tag insertion lines (n=2165) was generated by in planta transformation. Whole plants were vacuum-infiltrated in a suspension of Agrobacterium carrying the pGKB5 tagging vector. The efficiency of transformation increased with addition of the surfactant Silwet L-77 (0.005% v/v) to the Agrobacterium suspension. Visual screens of the T-DNA lines identified two mutants with floral defects. Allelism tests suggested that a mutation in the GIGANTEA gene was responsible for the late-flowering phenotype of one of the mutants. Linkage analysis indicated that the GIGANTEA gene was tagged in this mutant. 
