
%0 Journal Article
%A Giménez-Espinosa, R. 
%A Prado, R. De 
%T Absorption, translocation and metabolism of pyridate in chickpea (<emph type="2">Cicer arietinum</emph>)
%J Functional Plant Biology
%D 1998/03/30
%V 25
%N 1
%P 105-110
%K tolerance, absorption, translocation, metabolism, N-glycoside, glutathione, cysteine, glutathione transferase.
%R https://doi.org/10.1071/PP97090
%U https://www.publish.csiro.au/paper/PP97090 
%X This work studied the tolerance mechanisms of chickpea (Cicer arietinum L.) to pyridate, a post-emergence herbicide used in other crops such as maize, peanut and wheat. Foliar absorption of pyridate in chickpea was limited and occurred mainly during the first 24 h after treatment. Over 83% of the herbicide absorbed remained in the treated leaf 72 h after treatment. Twelve hours after herbicide treatment, 50% of the total herbicide applied was converted to CL9673-N-Gly, an intermediate metabolite essential in the activation of the pyridazine ring for following conjugations. The N-glycoside was conjugated to form more-polar metabolites, C1 and C2: C1 was identified as CL9673-N-Gly- glutathione; and C2 was identified as CL9673-N-Gly-cysteine. C1 appeared 3 h after treatment and reached a maximum of around 40% of recovered radioactivity 72 h later, while C2 appeared 24 h after treatment and reached a maximum of 10% 72 h later. Chickpea glutathione transferases do not seem to be involved in the formation of C1, thus the final amount of this conjugate mainly depends on the amount of N-glycoside formation in vivo. 
