
TY  - JOUR
A1  - Caemmerer, Susanne von 
A1  - Ludwig, Martha 
A1  - Millgate, Anthony 
A1  - Farquhar, Graham D. 
A1  - Price, Dean 
A1  - Badger, Murray 
A1  - Furbank, Robert T. 
T1  - Carbon Isotope Discrimination during C<emph tye="8">4</emph> Photosynthesis: Insights from Transgenic Plants
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 1997
VL  - 24
IS  - 4
SP  - 487
EP  - 494
KW  - 
UR  - https://doi.org/10.1071/PP97031 
N2  - We have measured the discrimination against 13C during CO2 assimilation in Flaveria bidentis wild type plants and in transgenic Flaveria bidentis plants transformed (1) with an antisense RNA construct targeted to the nuclear encoded gene for the small subunit of Rubisco&#8212;these plants had reduced amounts of Rubisco, decreased CO2 assimilation rates and increased carbon isotope discrimination, which was also evident in the carbon isotope discrimination of leaf dry matter; and (2) transformed with the mature coding region of carbonic anhydrase, CA, from tobacco (Nicotiana tabacum) in the sense direction under the control of the cauliflower mosaic virus 35S promoter&#8212;these plants had slightly increased CA activity in the mesophyll as well as a 2&#8211;4-fold increase in CA activity in the bundle-sheath cells. The introduction of tobacco CA manifested itself by a reduction in CO2 assimilation rate and an increase in carbon isotope discrimination. We suggest that the increased carbon isotope discrimination is a result of increased bicarbonate leakage out of the bundle sheath. 
ER  -

