
TY  - JOUR
A1  - Sültemeyer, Dieter 
A1  - Klughammer, Barbara 
A1  - Ludwig, Martha 
A1  - Badger, Murray R. 
A1  - Price, G. Dean 
T1  - Random Insertional Mutagenesis Used in the Generation of Mutants of the Marine Cyanobacterium <emph type="2">Synechococcus</emph> sp. Strain PCC7002 With an Impaired CO<sub>2</sub> Concentrating Mechanism
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 1997
VL  - 24
IS  - 3
SP  - 317
EP  - 327
KW  - 
UR  - https://doi.org/10.1071/PP96124 
N2  - Random insertional mutagenesis (gene tagging) has been used to generate ten kanamycin resistant mutants of the marine cyanobacterium, Synechococcus sp. strain PCC7002, defective in components of the CO2 concentrating mechanism (CCM). Three mutants had physiological characteristics consistent with defects in carboxysome functioning such as the ability to over-accumulate inorganic carbon (Ci) but make little use of this pool for photosynthesis. Another group of mutants could not survive at 20 mL CO2 L-1 (low Ci) even though they were able to induce high-affinity CO2 and HCO3- transport systems within 6 h of acclimation to low Ci, albeit at reduced maximum rates compared to wild type (WT) cells. A third class of mutant grew more slowly at 20 mL CO2 L-1 (pH 8.2) than WT cells and failed to grow at pH 7.0 under the same CO2 conditions. Besides inhibition of Ci transport rates in low-Ci cells and small internal Ci pools, these mutants had only partly developed a high-affinity HCO3- uptake system and had completely failed to induce a high-affinity CO2 transport system. Physiological characteristics of these mutants are discussed along with prospects for isolating the genes affected in these mutants. 
ER  -

