
TY  - JOUR
A1  - Mason, Michael Glenn 
A1  - Botella, José Ramón 
T1  - Identification and Characterisation of Two 1-Aminocyclopropane- 1-Carboxylate (ACC) Synthase cDNAs Expressed during Papaya (<emph type="2">Carica papaya</emph>) Fruit Ripening
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 1997
VL  - 24
IS  - 2
SP  - 239
EP  - 244
KW  - 1-Aminocyclopropane-1-carboxylate (ACC) synthase, ethylene, fruit ripening, Polymerase Chain Reaction (PCR), Carica papaya
UR  - https://doi.org/10.1071/PP96111 
N2  - The cloning and characterisation of two cDNAs (capacs1 and capacs2) encoding ACC synthase in papaya (Carica papaya L.) is described. capacs1 is 1104 bp long encoding 368 amino acids. capacs2 is 1098 bp long encoding 366 amino acids. The proteins encoded by both cDNAs contain the highly conserved active site of ACC synthases as well as 10 (capacs1) or 11 (capacs2) of the 12 amino acid residues conserved in most aminotransferases. Southern analyses indicate that capacs1 and capacs2 are present in the papaya genome as single copy genes. Both genes show very different expression patterns during fruit ripening. capacs1 mRNA levels are high in mature green fruits, decreasing steadily during ripening, whereas capacs2 mRNA levels are undetectable in mature green fruits but experience a dramatic increase with the onset of ripening and remain high throughout the various stages of ripening. 
ER  -

