
TY  - JOUR
A1  - Yu, Chih-Wen 
A1  - Murphy, Terence M. 
A1  - Sung, Wei-Wen 
A1  - Lin, Chin-Ho 
T1  - H<emph type="8">2</emph>O<emph type="8">2</emph> treatment induces glutathione accumulation and chilling tolerance in mung bean
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 2002
VL  - 29
IS  - 9
SP  - 1081
EP  - 1087
KW  - acclimation, buthionine sulfoximine (BSO), chilling, glutathione, hydrogen peroxide (H2O2), mung bean.
UR  - https://doi.org/10.1071/PP01264 
N2  - Mung bean (Vigna radiata L. cv. TN5, a chilling-sensitive cultivar) was employed to evaluate the importance of glutathione in hydrogen peroxide (H2O2)-induced chilling tolerance. Seeds germinated at 25&#176;C for 7 d were subjected to different periods of chilling treatment, prior to analysis of the glutathione contents of their leaves. In a comparison of acclimation temperatures from 2-12&#176;C, it was found that an 8&#176;C acclimation for 36 h induced a 5.7-fold increase, the highest glutathione level among the temperatures tested. Seedlings acclimated at 8&#176;C for 36 h showed 97% survival after a 36-h, 4&#176;C chilling stress, compared with 33% survival of non-acclimated plants. Pretreatment with 200 mM H2O2 for 12 h before a 36-h, 4&#176;C chilling treatment increased glutathione levels by 30% and reduced electrolyte leakage to 43%, relative to the untreated control. Treated seedlings also showed a survival rate of 71% after the same chilling treatment. Application of 1 mM buthionine sulfoximine, a specific inhibitor of glutathione synthesis, reversed the protection against chilling stress provided to seedlings either by acclimation at 8&#176;C for 36 h or H2O2 pretreatment. The role of glutathione in chilling acclimation or H2O2-pretreatment-induced chilling tolerance is thus confirmed. 
ER  -

