
TY  - JOUR
A1  - Ohkawa, Hiroshi 
A1  - Sonoda, Masatoshi 
A1  - Hagino, Natsu 
A1  - Shibata, Mari 
A1  - Pakrasi, Himadri B. 
A1  - Ogawa, Teruo 
T1  - Functionally distinct NAD(P)H dehydrogenases and their membrane localization in <emph type="2">Synechocystis</emph> sp. PCC6803
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 2002
VL  - 29
IS  - 3
SP  - 195
EP  - 200
KW  - CO2 uptake, cyanobacterium, cytoplasmic membrane, NAD(P)H dehydrogenase, ndh, Synechocystis 6803, thylakoid membrane.
UR  - https://doi.org/10.1071/PP01180 
N2  - The type I NAD(P)H dehydrogenase complex (NDH-1) in cyanobacteria is involved in both respiratory and photosynthetic electron transport processes. NDH-1 is also essential for inorganic carbon transport. It has been postulated that NDH-1-dependent cyclic electron flow around PSI energizes CO2 uptake. The genome information of Synechocystis sp. PCC6803 has enabled us to provide an integrative view of the CO2 concentrating mechanism in this organism. In an attempt to dissect the role of the NDH-1 complex, we have constructed single and double mutants of Synechocystis 6803 by disrupting highly homologous ndhD genes in pairs, and have analysed the growth, CO2 uptake activities, and redox levels of P700 and the plastoquinone pool in these mutants under various conditions. We have also determined the membrane localization of this membrane protein. Our studies have revealed that: (i) mutations in ndh genes lead to inhibition of CO2 uptake, rather than HCO3&#8211; uptake; (ii) NDH-1 complexes are localized only in the thylakoid membrane; (iii) there are functionally distinct NDH-1 complexes in Synechocystis #6803. Based on these data, we propose a schematic view of the roles of different NDH-1 complexes in cyanobacteria. 
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