
TY  - JOUR
A1  - Poupard, Pascal 
A1  - Brunel, Nicole 
A1  - Leduc, Nathalie 
A1  - Viémont, Jean-Daniel 
A1  - Strullu, Désiré-Georges 
A1  - Simoneau, Philippe 
T1  - Expression of a <emph type="2">Bet v 1</emph> homologue gene encoding a PR 10 protein in birch roots: induction by auxin and localization of the transcripts by <emph type="2">in situ</emph> hybridization
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 2001
VL  - 28
IS  - 1
SP  - 57
EP  - 63
KW  - 
UR  - https://doi.org/10.1071/PP00040 
N2  - In birch roots (Betula pendula Roth), two members of the Bet v 1 gene family which encode PR 10 proteins have previously been characterized. One of these members, named Bet v 1-sc1, is significantly induced in response to biotic or abiotic factors. We have analysed the expression of Bet v 1-sc1 in birch roots treated either with 1 M indole-3-acetic acid (IAA) or 1 M kinetin using reverse transcription&#8211;polymerase chain reaction (RT&#8211;PCR), northern blotting and competitive PCR. High accumulation of the Bet v 1-sc1 transcripts was recorded only after auxin application, while kinetin had no effect. By in situ hybridization, we have investigated the localization of Bet v 1-sc1 mRNA in birch roots after induction of the gene by root treatment with 1 M IAA. Using root tip sections, we showed that Bet v 1-sc1 is significantly expressed in the apical meristem and the procambium. In sections taken in the zone producing lateral roots, the presence of Bet v 1-sc1 was found at sites of emerging secondary root primordia. This first report of localization of Bet v 1-sc1 expression suggests that this gene could be involved in the processes leading to lateral root initiation. 
ER  -

