
TY  - JOUR
A1  - Rae, Anne L. 
A1  - Jarmey, Janine M. 
A1  - Mudge, Stephen R. 
A1  - Smith, Frank W. 
T1  - Over-expression of a high-affinity phosphate transporter in transgenic barley plants does not enhance phosphate uptake rates
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 2004
VL  - 31
IS  - 2
SP  - 141
EP  - 148
KW  - Hordeum vulgare, ion transport, mineral nutrition.
UR  - https://doi.org/10.1071/FP03159 
N2  - Transgenic barley plants that over-express the gene encoding a phosphate transporter were generated and used to test the hypothesis that manipulation of transporters may lead to improved phosphate uptake by plant roots. Replicate T2 seedlings from a homozygous line with a single locus insertion were grown in dilute flow culture. The phosphate contents and uptake rates of these plants were compared with control transgenic and wild-type plants. When external phosphate concentration was maintained at 10 &#956;M, all plants including the transgenic over-expressing line displayed low rates of phosphate uptake and contained high levels of phosphate in the shoot tissue. When external phosphate concentration was maintained at 2 &#956;M, the uptake rates increased to a similar level in all plant lines. Three transgenic over-expressing lines were then grown in soil at a range of phosphate concentrations and the dry weights and total phosphorus contents of the shoots were measured and compared to a transgenic control line. The results showed that over-expression of the gene encoding a phosphate transporter did not improve the uptake of phosphate under any of the conditions tested. Transporter activity is likely to be influenced by post-transcriptional mechanisms and will require further investigation before this strategy can be applied to improving plant nutrition. 
ER  -

