
TY  - JOUR
A1  - Kanoun, Myriam 
A1  - Goulas, Philippe 
A1  - Bassères, Anne 
A1  - Biolley, Jean-Philippe 
T1  - Ozone-induced oxidation of Rubisco: from an ELISA quantification of carbonyls to putative pathways leading to oxidizing mechanisms
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 2002
VL  - 29
IS  - 11
SP  - 1357
EP  - 1363
KW  - ELISA, ozone stress, Phaseolus vulgaris, protein carbonyls, protein oxidation, Rubisco, ribulose-1,5-bisphosphate carboxylase / oxygenase.
UR  - https://doi.org/10.1071/FP02040 
N2  - In an attempt to detect a possible relationship between protein oxidation and ozone (O3) atmospheric concentration, we used a sensitive enzyme-linked immunosorbent assay (ELISA) method for measuring carbonyl formation in amino acid residues that constitute Rubisco (EC 4.1.1.39) small subunit (Rubisco-SSU). Using open-top chamber technology, bean plants (Phaseolus vulgaris L. cv. Bergamo) were exposed for 21 d (from emergence) to four different atmospheres characterized by average daylight O3 concentrations of 12, 70, 89 and 109 nL L&#8211;1. Rubisco-SSU extracted from primary leaves was fixed specifically on wells coated with anti-SSU antibodies. Aldehydes and ketones, previously derivatized with 2,4-dinitrophenylhydrazine (DNP), were quantified with anti-DNP antibodies conjugated with alkaline phosphatase. A significant positive O3 effect on carbonyl formation was detected, and the number of carbonyls was found to be linearly increased (r2=0.82) when plotted against increasing external O3 dose expressed as accumulated exposure over a threshold of 40 nL L&#8211;1 h (AOT40). Furthermore, these O3-induced oxidative modifications were connected with a significant reduction in the amount of native SSU that linearly decreased (r2=0.95) as AOT40 increased from 0 to about 8295 nL L&#8211;1 h. A possible pathway leading to oxidation of Rubisco is proposed, with special reference to O3 reactivity. 
ER  -

