Photosynthesis-related physiology and metabolomics responses of Polygonum lapathifolium in contrasting manganese environments
Yongsen Wang A B , Xiaojin Guan A B , Zongbao Liu A B , Yi Li A C , Fangming Yu A C * and Kehui Liu
A B *
+ Author Affiliations
- Author Affiliations
A Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, 541004 Guilin, China.
B Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in the Lijiang River Basin, Guangxi Normal University, 541004 Guilin, China.
C College of Environment and Resource, Guangxi Normal University, Guilin, China.
Functional Plant Biology 50(3) 242-255 https://doi.org/10.1071/FP22208
Submitted: 9 September 2022 Accepted: 16 November 2022 Published: 20 December 2022
© 2023 The Author(s) (or their employer(s)). Published by CSIRO Publishing
Abstract
Manganese (Mn) plays an essential role in plant growth; however, excessive Mn is toxic to plants. Polygonum lapathifolium Linn. was tested as a novel Mn-hyperaccumulating species in our previous study, but the underlying mechanisms of this hyperaccumulation are poorly understood. A hydroponic experiment with (8 mmol L−1) and without additional Mn (CK) was established to explore the possible mechanisms through the effects on photosynthesis-related physiological characteristics and metabolomics. The results showed that additional Mn increased plant biomass, photosynthesis, and stomatal conductance related to increases in the effective photochemical quantum yield of photosystem II and relative electron transport rate (P < 0.05). The results from liquid chromatography–mass spectrometry revealed 56 metabolites differentially accumulated between the plants composing these two groups. Metabolites were enriched in 20 metabolic pathways at three levels (environmental information processing, genetic information processing, and metabolism), of which five metabolic pathways were associated with significant or extremely significant changes (P < 0.05). These five enriched pathways were ABC transporters (environmental information processing), aminoacyl-tRNA biosynthesis (genetic information processing), biosynthesis of amino acids, d-arginine and d-ornithine metabolism, and arginine biosynthesis (metabolism). Flavonoids may play a key role in Mn tolerance, as they accumulated more than 490-fold, and the relationship between flavonoids and Mn tolerance needs to be studied in the future.
Keywords: chlorophyll, flavonoid, fluorescence, liquid chromatography, manganese stress, mass spectrometry, metabolomics, photosynthesis, physiology, superaccumulator.
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