Contribution of K solubilising bacteria (Burkholderia sp.) promotes tea plant growth (Camellia sinesis) and leaf polyphenols content by improving soil available K level
Xianchen Zhang
A # , Ningning Wang A # , Mengmeng Hou B # , Honghong Wu C , Hong Jiang D , Ziwen Zhou A , Na Chang A , Qianqian Wang A , Xiaochun Wan A , Jiayue Jiang A , Zhougao Shen A and Yeyun Li A *
+ Author Affiliations
- Author Affiliations
A State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China.
B Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.
C College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
D Anhui Keemun Black Tea Industry Co., Ltd., Huangshan City, Anhui Province, China.
* Correspondence to: Liyeyun360@163.com
# These authors contributed equally to this paper
Handling Editor: Fanrong Zeng
Functional Plant Biology 49(3) 283-294 https://doi.org/10.1071/FP21193
Submitted: 15 April 2021 Accepted: 21 December 2021 Published: 1 February 2022
© 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing
Abstract
K+ availability is important for growth and quality of tea (Camellia sinesis L.). K solubilising bacteria convert insoluble K to available K. This study was conducted to screen K solubilising bacteria isolated from tea rhizosphere soil in Qimen county, Anhui province, China. The maximum K solubilisation colony (the ratio of diameter halo/colony was 2.54) was identified as Burkholderia sp. (storage number: M2021105) by biochemistry and molecular analysis. Pot experiments (Laterite) showed that the inoculation of Burkholderia sp. significantly improved tea plant height (Zhongcha108, 1 year old) and total polyphenols content by 21.14% and 21.58% compared with the control, respectively. Higher polyphenol level promoted the formation of theaflavin in the fermentation experiments. Further experiments showed that tartaric acid and pryuvic acid produced by Burkholderia sp. are important components associated with K solubilisation in vitro. Burkholderia sp. significantly increased soil available K by 15.12%; however, there was no significant difference in available N and P, and Cu, Mg, Zn and Ca compared with the control. K content in inoculated tea roots and leaves was significantly higher (50% and 10%, respectively) than the control. Compared with the control, exogenous supply of 60 mg kg−1 K significantly increased levels of polyphenol (53.97%), theaflavin (16.31%), theaflavin-3-gallate (20%), theaflavin 3′-gallic acid ester (32.24%) and theaflavin 3,3′-gallic acid ester (40.95%). Due to its ability to enable higher available soil K, ur study indicated that Burkholderia sp. have potential to increase total polyphenols content be a bio-inoculant for biofortification of tea.
Keywords: K level, K solubilisation bacteria, polyphenol, pot experiment, pryuvic acid, tartaric acid, tea, theaflavin.
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