New insights into the effect of NdhO levels on cyanobacterial cell death triggered by high temperature
Jiaohong Zhao A , Yuanyuan Jiang A , Yuhao Tian A , Jun Mao A , Lanzhen Wei A B and Weimin Ma
A B
+ Author Affiliations
- Author Affiliations
A Shanghai Key laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, China.
B Corresponding author. Email: wma@shnu.edu.cn; weilz@shnu.edu.cn
Functional Plant Biology - https://doi.org/10.1071/FP21097
Submitted: 1 April 2021 Accepted: 5 August 2021 Published online: 25 August 2021
Abstract
NdhO, a regulatory oxygenic photosynthesis-specific subunit, is close to the ferredoxin-binding site of cyanobacterial NDH-1, and its levels are negatively associated with the rates of cyclic electron transfer around PSI mediated by NDH-1 (NDH-CET). However, the effect of NdhO levels on cyanobacterial cell death triggered by high temperature remains elusive. Here, our results uncovered a synergistic effect of NdhO levels on the cell death and reactive oxygen species (ROS) accumulation when cyanobacterial cells grown at 30°C for 1 day were transferred to 45°C for 2 days. Such synergistic effect was found to be closely associated with the activities of NDH-CET and CO2 assimilation during high temperature. Collectively, we propose that the effect of NdhO levels on the cyanobacterial cell bleaching and cell death triggered by high temperature is a result of influencing production of ROS by NDH-CET, which is considered to be vital to balance the ATP/NADPH ratio and improve the Calvin-Benson cycle.
Keywords: NdhO, high temperature, cyclic electron transfer, CO2 fixation, reactive oxygen species, cell death, Synechocystis.
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