Regulation of salt-stressed sunflower (Helianthus annuus) seedling’s water status by the coordinated action of Na+/K+ accumulation, nitric oxide, and aquaporin expression
Archana Kumari A and Satish C. Bhatla
A B
+ Author Affiliations
- Author Affiliations
A Laboratory of Plant Physiology and Biochemistry, Department of Botany, University of Delhi, Delhi-11007, India.
B Corresponding author. Email: bhatlasc@gmail.com
Functional Plant Biology 48(6) 573-587 https://doi.org/10.1071/FP20334
Submitted: 28 October 2020 Accepted: 25 December 2020 Published: 25 January 2021
Abstract
Among abiotic stresses, salt stress is a major threat to crop production all over the world. Present work demonstrates the profuse accumulation of Na+ in 2-day-old, dark-grown sunflower (Helianthus annuus L.) seedlings roots in response to salt stress (NaCl). The pattern of K+ accumulation in response to salt stress is similar to that of Na+ but on relatively lower scale. Application of nitric oxide (NO) donor (DETA) scales down Na+ accumulation in salt-stressed seedlings. The impact of NO donor on K+ accumulation is, however, different in control and salt-stressed seedling roots. In control seedlings, it enhances K+ accumulation, whereas, it gets reduced in salt-stressed seedlings. Specialised channels called ‘aquaporins’ (AQPs) play a major role maintaining the water status and transport across plant parts under salt-stress. Thus, accumulation of plasma-membrane intrinsic proteins (PIPs) and tonoplast-intrinsic proteins (TIPs), localised on plasma-membrane and vacuolar-membrane, respectively was undertaken in 2-day-old, dark-grown seedling roots. Salt stress increased the abundance of these isoforms, whereas, NO application resulted in decreased accumulation of PIP2 and TIP1. PIP1 and TIP2 isoforms remained undetectable. Present work thus, puts forward a correlation between AQP expression and ions (Na+ and K+) homeostasis in response to salt stress and NO.
Keywords: aquaporin, ion homeostasis, nitric oxide, plasma membrane intrinsic proteins, salt stress, tonoplast membrane intrinsic proteins, sunflower, nitric oxide.
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