OsLEA1a overexpression enhances tolerance to diverse abiotic stresses by inhibiting cell membrane damage and enhancing ROS scavenging capacity in transgenic rice
Zhaodan Wang A , Qian Zhang B , Juan Qin A , Guosheng Xiao A , Shanshan Zhu B and Tingzhang Hu
A B C
+ Author Affiliations
- Author Affiliations
A Engineering Technology Research Centre of Characteristic Biological Resources in Northeast of Chongqing, College of Biology and Food Engineering, Chongqing Three Gorges University, Chongqing 404120, China.
B Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing 400030, China.
C Corresponding author. Email: tzhu2002@foxmail.com
Functional Plant Biology 48(9) 860-870 https://doi.org/10.1071/FP20231
Submitted: 4 August 2020 Accepted: 11 March 2021 Published: 6 April 2021
Abstract
Late embryogenesis abundant (LEA) proteins are involved in diverse abiotic stresses tolerance in many different organisms. Our previous studies have shown that the heterologous expression of OsLEA1a interfered with the resistance of Escherichia coli to abiotic stresses. However, in the present study, based on growth status and physiological indices of rice plant, the overexpression of OsLEA1a in rice conferred increased resistance to abiotic stresses compared with the wild-type (WT) plants. Before applying abiotic stresses, there were no significant differences in physiological indices of rice seedlings. After NaCl, sorbitol, CuSO4 and H2O2 stresses, the transgenic lines had lower relative electrical conductivity, malondialdehyde and lipid peroxidation, greater the contents of proline, soluble sugar and glutathione, and higher the activities of superoxide dismutase, catalase and peroxidase than the WT plants. The results indicate that the OsLEA1a gene is involved in the protective response of plants to various abiotic stresses by inhibiting cell membrane damage and enhancing reactive oxygen species scavenging capacity. It was speculated that post-translational modification causes OsLEA1a functional differences in E. coli and rice. The present study shows that OsLEA1a could be a useful candidate gene for engineering abiotic stress tolerance in cultivated plants.
Keywords: abiotic resistance, genetic engineering, late embryogenesis abundant proteins, LEA, Oryza sativa, OsLEA1a gene, rice, superoxide levels.
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