Metabolomics reveals the influences of smoke-water and karrikinolide on the biosynthesis of flavonoids and terpenoids in Salvia miltiorrhiza
Hui Sun A , Qian Liu B , Jie Zhou
A D and Lanping Guo C D
+ Author Affiliations
- Author Affiliations
A School of Biological Science and Technology, University of Jinan, Jinan, 250022, China.
B College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, 250355, China.
C State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
D Corresponding authors. Email: zhoujie8761@163.com; guolanpingabc@126.com
Functional Plant Biology - https://doi.org/10.1071/FP20172
Submitted: 18 June 2020 Accepted: 21 October 2020 Published online: 27 November 2020
Abstract
Metabolomics was used to study the influences of smoke-water (SW) and karrikinolide (KAR1) on the biosynthesis of flavonoids and terpenoids in Salvia miltiorrhiza Bunge. The results showed that a total of 178 and 199 differential metabolites were obtained in SW and KAR1, respectively, compared to the control. The differential metabolites were assigned to the corresponding metabolic pathways. The results indicated that some metabolic pathways in treatments of SW and KAR1 overlapped, suggesting that treatments of SW and KAR1 showed similar effects on the metabolic mechanism of S. miltiorrhiza. To obtain a clear overview of changes in metabolic regulation, TCA cycle, glycolytic pathway, biosynthesis of flavonoids and terpenoids and amino acids metabolism pathway were mapped into a network. We found that treatments with SW and KAR1 could significantly promote the biosynthesis of flavonoids and terpenoids in S. miltiorrhiza. This study could help us better understand the influences of SW and KAR1 on secondary metabolites and their underlying mechanism.
Keywords: danshen, flavonoids, karrikinolide, metabolites, metabolomics, Salvia miltiorrhiza Bunge, smoke-water, terpenoids.
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