OsCER1 regulates humidity-sensitive genic male sterility through very-long-chain (VLC) alkane metabolism of tryphine in rice
Erdong Ni A B C * , Li Deng A B * , Huiqiong Chen A B , Jianwen Lin A B , Jiamin Ruan A B , Zhenlan Liu A B , Chuxiong Zhuang A B and Hai Zhou
A B D
+ Author Affiliations
- Author Affiliations
A State Key Laboratory for Conservation and Utilisation of Subtropical Agro-bioresources, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
B Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, Guangdong 510642, China.
C Tea Research Institute, Guangdong Academy of Agricultural Sciences; Guangdong Key Laboratory of Tea Plant Resources Innovation & Utilization, Guangzhou, Guangdong 510640, China.
D Corresponding author. Email: haizhou@scau.edu.cn
Functional Plant Biology 48(5) 461-468 https://doi.org/10.1071/FP20168
Submitted: 12 June 2020 Accepted: 25 November 2020 Published: 25 January 2021
Abstract
Humidity-sensitive genic male sterility (HGMS) is a novel type of environment-sensitive male sterility (EGMS) which plants are male sterile at low humidity and male fertile at high humidity. Previous studies have revealed that OsCER1 contributes to very-long-chain (VLC) alkanes biosynthesis in rice (Oryza sativa L.). Here, applying the CRISPR/Cas9 technique, we obtained two independent OsCER1 knockout lines (OsCER1Cas). Both OsCER1Cas lines exhibited HGMS. Mutant pollen showed defects in adhesion and germination on stigmas at low humidity, whereas high humidity enhanced the pollen germination rate. Transmission electron microscopy (TEM) observations of mutant pollen revealed abnormal tryphine structure, potentially representing the basis of HGMS. Furthermore, co-pollination with mixed OsCER1Cas mutant and maize (Zea mays L.) pollen could rescue the fertility of the mutant, thereby establishing the key role of tryphine in germination on stigmas. OsCER1 knockout might affect VLC alkane metabolism and therefore alter the lipid composition of tryphine. It could lead to the defects in pollen grain adhesion, hydration and germination, resulting in HGMS. This work identified the mechanism of HGMS induced by VLC alkanes in rice and the generality of tryphine in different species of Gramineae.
Keywords: environment-sensitive male sterility, humidity, very-long-chain (VLC) alkanes, OsCER1.
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