Diurnal changes of the ascorbate-glutathione cycle components in wheat genotypes exposed to drought
Durna R. Aliyeva
A , Lala M. Aydinli A , Ismayil S. Zulfugarov A B C and Irada M. Huseynova A D
+ Author Affiliations
- Author Affiliations
A Institute of Molecular Biology and Biotechnologies, Azerbaijan National Academy of Sciences, 11 Izzat Nabiyev Str., Baku AZ 1073, Azerbaijan.
B Department of Integrated Biological Science and Department of Molecular Biology, Pusan National University, Busan 46241, Korea.
C Department of Biology, North-Eastern Federal University, 58 Belinsky Str., Yakutsk 677-027, Republic of Sakha (Yakutia), Russian Federation.
D Corresponding author. Email: i.huseynova@imbb.science.az
This paper originates from a presentation at the 10th International Photosynthesis and Hydrogen Energy Research for Sustainability Meeting held in St Petersburg, Russia, 23–28 June 2019.
Functional Plant Biology 47(11) 998-1006 https://doi.org/10.1071/FP19375
Submitted: 24 December 2019 Accepted: 17 April 2020 Published: 22 June 2020
Abstract
The ascorbate-glutathione (AsA-GSH) cycle is a major pathway of H2O2 scavenging in plants. The effect of diurnal variations in hydrogen peroxide (H2O2) content, the intensity of lipid peroxidation (malondialdehyde, MDA), photosynthesis, antioxidants and antioxidative enzyme activities involved in AsA-GSH metabolism has been studied comparatively in leaves of durum (Triticum durum Desf.) and bread (Triticum aestivum L.) wheat genotypes exposed to soil drought. Drought stress caused an increase in the content of H2O2, MDA, alterations in the activities of AsA-GSH cycle enzymes and quantitative changes in AsA and GSH content during the day. PSII efficiency was significantly lower in the control and drought exposed leaves at the highest temperature in the afternoon. The ascorbate peroxidase activity was found to increase and ascorbic acid amount decreased with increasing temperature during the day. Further, the glutathione amount and glutathione reductase activity increased at the expense of the regeneration of the oxidised form of glutathione. Our results revealed that wheat can tolerate drought stress by enhancing the antioxidant enzyme activities and alteration of the concentration of ascorbate and glutathione.
Additional keywords: ascorbate peroxidase, drought, glutathione reductase, Triticum durum, Triticum aestivum.
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