Ectopic expression of a R2R3 MYB transcription factor of dove tree (Davidia involucrata) aggravates seed abortion in Arabidopsis thaliana
Jian Li A B , Tian Chen A , Fengzhen Huang A B , Penghui Dai A B , Fuxiang Cao B C and Meng Li
A B D
+ Author Affiliations
- Author Affiliations
A College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China.
B Hunan Research Center of Engineering Technology for Utilisation of Environmental and Resources Plant, Changsha 410004, China.
C College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410004, China.
D Corresponding author. Email: limeng0422@foxmail.com
Functional Plant Biology 47(5) 454-463 https://doi.org/10.1071/FP19317
Submitted: 4 November 2019 Accepted: 12 December 2019 Published: 26 March 2020
Abstract
Serious seed abortion of dove tree (Davidia involucrate Baill.) is one of the critical factors leading to the low fecundity of this species. Seed abortion is a complicated process and various factors have been verified to synergistically determine the fate of seeds. To reveal the mechanism of seed abortion in D. involucrata, we performed transcriptome analysis in normal and abortive seeds of D. involucrata. According to the transcriptome data, we noticed that most of the genes encoding a MYB transcription factor were predominantly expressed in abortive seeds. Among these, a gene named DiMYB1 was selected and its function was validated in this study. Overexpression of DiMYB1 resulted in obviously reduced viability of transgenic seeds and seedlings, and caused a significantly higher seed abortion rate. The vegetative growth of transgenic plants was hindered, resulting in an earlier flowering time. In addition, colour changes occurred in transgenic plants. Some transgenic sprouts, stems and pods appeared purple instead of green in colour. Our finding demonstrated that DiMYB1 participates in multiple plant developmental processes, especially in seed development in Arabidopsis thaliana (L.) Heynh., which indicated the similar role of this gene in D. involucrata.
Additional keywords: anthocyanin, seed development, transgenic plants, transcriptome analysis.
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