Glucosinolate profile and Myrosinase gene expression are modulated upon Plasmodiophora brassicae infection in cabbage
Md. Abdul Kayum A , Ujjal Kumar Nath A , Jong-In Park A C , Mohammad Rashed Hossain A , Hoy-Taek Kim A , Hye-Ran Kim B and Ill-Sup Nou
A C
+ Author Affiliations
- Author Affiliations
A Department of Horticulture, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922, South Korea.
B Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South Korea.
C Corresponding authors. Email: nis@sunchon.ac.kr; jipark@sunchon.ac.kr
Functional Plant Biology 48(1) 103-118 https://doi.org/10.1071/FP19278
Submitted: 3 October 2019 Accepted: 20 July 2020 Published: 12 August 2020
Abstract
Clubroot is a devastating disease of Brassicaceae caused by the biotrophic protist Plasmodiophora brassicae. The progression of clubroot disease is modulated by the glucosinolate (GSL) profile of the host plant. GSL is hydrolysed by the enzyme myrosinase upon cell disruption and gives rise to metabolites like isothiocyanate, nitriles, thiocyanates, epithionitriles and oxazolidines. Some of these metabolites play important roles in the plant’s defence mechanism. We identified 13 Myrosinase (Myro) and 28 Myrosinase-Binding Protein-like (MBP) genes from Brassica oleracea L. using a comparative genomics approach and characterised them through in silico analyses. We compared the expression patterns of these genes in a clubroot-susceptible line and a resistant line following inoculation with P. brassicae. Two BolMyro and 12 BolMBP genes were highly expressed in the susceptible line, whereas only one BolMyro and five BolMBP genes were highly expressed in the resistant line. Principal component analysis confirmed that specific GSL profiles and gene expression were modulated due to pathogen infection. Plants with higher levels of neoglucobrassicin, glucobrassicin and methooxyglucobrassicin produced disease symptoms and formed galls, whereas, plants with higher levels of sinigrin, hydroxyglucobrassicin and progoitrin produced less symptoms with almost no galls. Our results provide insights into the roles of Myro and MBP genes in GSL hydrolysis during P. brassicae infection, which will help for developing clubroot resistant cabbage lines.
Additional keywords: Brassica spp, disease resistance, gene expression, glucosinolate.
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