Characterisation of a rice vacuolar invertase isoform, OsINV2, for growth and yield-related traits
Shamitha R. Morey A , Tatsuro Hirose B D , Yoichi Hashida A D , Akio Miyao C , Hirohiko Hirochika C , Ryu Ohsugi A , Junko Yamagishi A and Naohiro Aoki
A E
+ Author Affiliations
- Author Affiliations
A Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
B Central Region Agricultural Research Center, NARO, 1-2-1 Inada, Joetsu, Niigata, 943-0193, Japan.
C Advanced Genomics Breeding Section, Institute of Crop Science, NARO, 2-1-2, Kannondai, Tsukuba, Ibaraki, 305-8518, Japan.
D Present address: Faculty of Agriculture, Takasaki University of Health and Welfare, 54 Nakaorui-machi, Takasaki, Gunma, 370-0033, Japan.
E Corresponding author. Email: aaokin@mail.ecc.u-tokyo.ac.jp
Functional Plant Biology 46(8) 777-785 https://doi.org/10.1071/FP18291
Submitted: 30 October 2018 Accepted: 06 April 2019 Published: 2 May 2019
Abstract
OsINV2, a rice vacuolar invertase isoform, was assessed for its functional roles in plant growth and development with key focus on its agronomic traits such as grain weight, grain filling percentage, grain number and dry weights at various stages until harvest. Lack of differences between the wild-type and the mutants with respect to any of the aforementioned traits tested revealed a possibility of functional compensation of OsINV2 in the mutants conceivably by its isoform. This was confirmed by OsINV2 promoter::GUS studies, where its spatial and temporal expression in the panicle elongation stages showed that although OsINV2 expression was observed from the stage with young panicles ~1 cm in length to the flag leaf stage, significant differences with respect to panicle and spikelet phenotypes between the wild-type and the mutant were not present. However, complement lines displaying an overexpression phenotype of OsINV2 possessed a higher stem non-structural carbohydrate content under both monoculm and normal tillering conditions. A trade-off between the spikelet number and grain weight in the complement lines grown under monoculm conditions was also observed, pointing towards the necessity of OsINV2 regulation for grain yield-related traits.
Additional keywords: carbohydrate metabolism, grain size, grain weight, non-structural carbohydrates, soluble acid invertase, Tos17 retrotransposon, vacuolar invertase, yield.
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