Involvement of OpsLTP1 from Opuntia streptacantha in abiotic stress adaptation and lipid metabolism
Mario Rojas A , Francisco Jimenez-Bremont B , Claudia Villicaña C , Laura Carreón-Palau A , Bertha Olivia Arredondo-Vega A and Gracia Gómez-Anduro A D
+ Author Affiliations
- Author Affiliations
A Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Av. Instituto Politécnico Nacional 195, Col. Playa Palo de Santa Rita Apdo, Postal 128, 23096 La Paz, B.C.S., México.
B Instituto Potosino de Investigación Científica y Tecnológica. Camino a la Presa San José 2055, Col. Lomas 4 sección CP. 78216, San Luis Potosí, S.L.P., México.
C CONACYT-Centro de Investigación en Alimentación y Desarrollo, A. C. Carretera a Eldorado Km. 5.5, Apartado Postal 32-A. C. P. 80110, Culiacán, Sinaloa, México.
D Corresponding author. Email: ggomez@cibnor.mx
Functional Plant Biology 46(9) 816-829 https://doi.org/10.1071/FP18280
Submitted: 23 October 2018 Accepted: 27 April 2019 Published: 29 May 2019
Abstract
Plant lipid transfer proteins (LTPs) exhibit the ability to transfer lipids between membranes in vitro, and have been implicated in diverse physiological processes associated to plant growth, reproduction, development, biotic and abiotic stress responses. However, their mode of action is not yet fully understood. To explore the functions of the OpsLTP1 gene encoding a LTP from cactus pear Opuntia streptacantha Lem., we generated transgenic Arabidopsis thaliana (L.) Heynh. plants to overexpress OpsLTP1 and contrasted our results with the loss-of-function mutant ltp3 from A. thaliana under abiotic stress conditions. The ltp3 mutant seeds showed impaired germination under salt and osmotic treatments, in contrast to OpsLTP1 overexpressing lines that displayed significant increases in germination rate. Moreover, stress recovery assays showed that ltp3 mutant seedlings were more sensitive to salt and osmotic treatments than wild-type plants suggesting that AtLTP3 is required for stress-induced responses, while the OpsLTP1 overexpressing line showed no significant differences. In addition, OpsLTP1 overexpressing and ltp3 mutant seeds stored lower amount of total lipids compared with wild-type seeds, showing changes primarily on 16C and 18C fatty acids. However, ltp3 mutant also lead changes in lipid profile and no over concrete lipids which may suggest a compensatory activation of other LTPs. Interestingly, linoleic acid (18:2ω6) was consistently increased in neutral, galactoglycerolipids and phosphoglycerolipids of OpsLTP1 overexpressing line indicating a role of OpsLTP1 in the modulation of lipid composition in A. thaliana.
Additional keywords: cactus pear, lipid seed composition, osmotic stress, salt stress, transgenic and mutant Arabidopsis.
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