Exogenous application of methyl jasmonate induces defense response and develops tolerance against mungbean yellow mosaic India virus in Vigna mungo
Nibedita Chakraborty A and Jolly Basak
A B
+ Author Affiliations
- Author Affiliations
A Department of Biotechnology, Visva-Bharati University, Santiniketan, 731235, India.
B Corresponding author. Email: jolly.basak@visva-bharati.ac.in
Functional Plant Biology 46(1) 69-81 https://doi.org/10.1071/FP18168
Submitted: 26 June 2018 Accepted: 29 August 2018 Published: 18 October 2018
Abstract
Vigna mungo (L.) Hepper is an economically important leguminous crop in south-east Asia. However, its production is severely affected by Mungbean yellow mosaic India virus (MYMIV). It is well established that methyl jasmonate (MeJA) is effective in inducing resistance against pathogens in several plants. To assess the role of MeJA in developing MYMIV tolerance in V. mungo, we analysed time-dependent biochemical and molecular responses of MYMIV susceptible V. mungo after exogenous application of different MeJA concentrations, followed by MYMIV infection. Our analysis revealed that exogenous application of different concentrations of MeJA resulted in decreased levels of malondialdehyde with higher membrane stability index values in MYMIV susceptible V. mungo, suggesting the protective role of MeJA through restoring the membrane stability. Moreover, the level of expression of different antioxidative enzymes revealed that exogenous MeJA is also very effective in ROS homeostasis maintenance. Enhanced expressions of the defence marker genes lipoxygenase and phenylalanine ammonia-lyase and the reduced expression of the MYMIV coat-protein encoding gene in all MeJA treated plants post MYMIV infection revealed that exogenous application of MeJA is effective for MYMIV tolerance in V. mungo. Our findings provide new insights into the physiological and molecular mechanisms of MYMIV tolerance in Vigna induced by MeJA.
Additional keywords: MeJA, MYMIV, plant defence, tolerance, Vigna mungo.
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