Grapevine fanleaf virus affects grape (Vitis vinifera) berry anthocyanin content via the transcriptional regulation of anthocyanin biosynthetic genes
Maja Rupnik-Cigoj A B C F , Anastazija Jež-Krebelj A B C , Simone D. Castellarin D , Kajetan Trošt A , Paolo Sivilotti A E and Maruša Pompe-Novak A B
+ Author Affiliations
- Author Affiliations
A School for Viticulture and Enology, University of Nova Gorica (UNG), Glavni trg 8, 5271 Vipava, Slovenia.
B Department of Biotechnology and Systems Biology, National Institute of Biology (NIB), Večna Pot 111, 1000 Ljubljana, Slovenia.
C Regional Development Agency of Northern Primorska Ltd, Nova Gorica (RRA SP), Trg Edvarda Kardelja 3, 5000 Nova Gorica.
D Wine Research Centre, The University of British Columbia, 2205 East Mall, Vancouver, BC, Canada.
E University of Udine, Department of Agricultural, Food, Environmental and Animal Sciences, via delle Scienze 206, Udine, Italy.
F Corresponding author. Email: maja.cigoj1@gmail.com
Functional Plant Biology 45(7) 771-782 https://doi.org/10.1071/FP18014
Submitted: 14 April 2017 Accepted: 24 January 2018 Published: 13 March 2018
Abstract
Grapevine fanleaf virus (GFLV) causes grapevine fanleaf degeneration, one of the oldest known viral diseases of grapevines. The virus has been found in all winegrowing regions around the world. In the seasons 2011–12 a comparison between field grown GFLV-infected and healthy grapevines was conducted for the cultivars Schioppettino in North-Eastern Italy and Refošk in South-Western Slovenia. Our research showed that GFLV infection caused a drop of the yield due to reduction of both cluster weight and berry weight. Besides the yield, the berry composition was also affected; in detail, anthocyanin concentration increased in both varieties but significantly only in the case of Schioppettino. Upregulation of the F3ʹ5ʹH gene and downregulation of F3ʹH gene in the berries of GFLV infected vines compared with the ones of healthy control vines resulted in modified proportions between di- and tri- hydroxylated or methylated derivatives of anthocyanins. The F3H1 gene was identified to be the most strongly regulated gene of the flavonoid biosynthetic pathway by GFLV infection, indicating its important role in increasing anthocyanin concentration in grapes of GFLV infected vines as compared with healthy controls.
Additional keywords: anthocyanins, gene expression, grape quality, yield.
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