Melatonin alleviates aluminium toxicity through modulating antioxidative enzymes and enhancing organic acid anion exudation in soybean
Jiarong Zhang A * , Bingjie Zeng A * , Yawen Mao A , Xiangying Kong A C , Xinxun Wang E , Ye Yang A , Jie Zhang D , Jin Xu B , Zed Rengel E and Qi Chen A F
+ Author Affiliations
- Author Affiliations
A Faculty of Life Science and Technology, Kunming University of Science and Technology, Jingming South Road, Kunming, 650500, China.
B Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, Mengla, Yunnan, 666303, China.
C Faculty of Architecture and City Planning, Kunming University of Science and Technology, Jingming South Road, Kunming, 650500, China.
D Biotechnology and Germplasm Resource Institute, Yunnan Academy of Agricultural Sciences, Yunnan Province Key Laboratory of Agricultural Biotechnology, Kunming 650223, China.
E UWA School of Agriculture and Environment, Faculty of Science, The University of Western Australia, 35 Stirling Highway, Perth WA 6009, Australia.
F Corresponding author. Email: chenq0321@163.com
Functional Plant Biology 44(10) 961-968 https://doi.org/10.1071/FP17003
Submitted: 3 January 2017 Accepted: 27 May 2017 Published: 23 June 2017
Abstract
Aluminium (Al) toxicity is a major chemical constraint limiting plant growth and production on acidic soils. Melatonin (N-acetyl-5-methoxytryptamine) is a ubiquitous molecule that plays crucial roles in plant growth and stress tolerance. However, there is no knowledge regarding whether melatonin is involved in plant responses to Al stress. Here, we show that optimal concentrations of melatonin could effectively ameliorate Al-induced phytotoxicity in soybean (Glycine max L.). The concentration of melatonin in roots was significantly increased by the 50 μM Al treatment. Such an increase in endogenous melatonin coincided with the upregulation of the gene encoding acetyltransferase NSI-like (nuclear shuttle protein-interacting) in soybean roots. Supplementation with low concentrations of melatonin (0.1 and 1 μM) conferred Al resistance as evident in partial alleviation of root growth inhibition and decreased H2O2 production: in contrast, high concentrations of melatonin (100 and 200 μM) had an opposite effect and even decreased root growth in Al-exposed seedlings. Mitigation of Al stress by the 1 μM melatonin root treatment was associated with enhanced activities of the antioxidant enzymes and increased exudation of malate and citrate. In conclusion, melatonin might play a critical role in soybean resistance to Al toxicity.
Additional keywords: Al toxicity, citrate, Glycine max, H2O2, malate, melatonin synthesis.
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