Aquaporin AtTIP5;1 as an essential target of gibberellins promotes hypocotyl cell elongation in Arabidopsis thaliana under excess boron stress
Yongqi Pang A B * , Jintong Li A * , Bishu Qi C D , Mi Tian A , Lirong Sun A , Xuechen Wang A and Fushun Hao A E
+ Author Affiliations
- Author Affiliations
A State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, College of Life Sciences, Henan University, Kaifeng 475004, P.R. China.
B Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, P.R. China.
C North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Agricultural University of Hebei, Baoding 071000, P.R. China.
D The National Police University for Criminal Justice, Baoding 071000, P.R. China.
E Corresponding author. Email: haofsh@henu.edu.cn
Functional Plant Biology 45(3) 305-314 https://doi.org/10.1071/FP16444
Submitted: 31 December 2016 Accepted: 29 August 2017 Published: 2 October 2017
Abstract
Aquaporins play essential roles in growth and development including stem elongation in plants. Tonoplast aquaporin AtTIP5;1 has been proposed to positively regulate hypocotyl elongation under high concentrations of boron (high-B) in Arabidopsis thaliana (L.) Heynh. However, the mechanism underlying this process remains unanswered. Here, we show that paclobatrazol, an inhibitor of GA biosynthesis, significantly suppressed the hypocotyl cell elongation of wild-type (WT) seedlings, and more strongly suppressed that of AtTIP5;1 overexpressors under high-B stress. Two AtTIP5;1 null mutants displayed arrested elongation of cells in the upper part of hypocotyls compared with the WT in the presence of high-B or GA3. Moreover, paclobatrazol treatment completely inhibited the increases in AtTIP5;1 transcripts induced by high-B, whereas GA3 application upregulated AtTIP5;1 expression in the WT. In addition, treatment with high-B remarkably elevated the expression levels of GA3ox1, GA20ox1 and GA20ox2 – key biosynthesis genes of GAs – in WT seedlings. The GA3 and GA4 content also increased in WT seedlings grown in MS medium containing high-B. Additionally, application of high-B failed to enhance AtTIP5;1 expression in the double mutant rga-24–gai-t6 of DELLA genes. Together, these results suggest that AtTIP5;1 is an essential downstream target of GAs. High-B induces the accumulation of GAs, which activates AtTIP5;1 through modulation of the DELLA proteins Repressor of ga1–3 and GA-insensitive, further promoting hypocotyl elongation in A. thaliana.
Additional keywords: AtTIP5;1 overexpressors, boron stress, DELLA, paclobatrazol.
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