Metabolomics analysis of postphotosynthetic effects of gaseous O2 on primary metabolism in illuminated leaves
Cyril Abadie A , Sophie Blanchet A B , Adam Carroll A and Guillaume Tcherkez A C
+ Author Affiliations
- Author Affiliations
A Research School of Biology, College of Medicine, Biology and Environment, Australian National University, Canberra, ACT 2601, Australia.
B Institute of Plant Science Paris-Saclay, UMR Université Paris-Sud-CNRS-INRA-Université Paris-Diderot-UEVE 1403, 91405 Orsay, France.
C Corresponding author. Email: guillaume.tcherkez@anu.edu.au
Functional Plant Biology 44(9) 929-940 https://doi.org/10.1071/FP16355
Submitted: 13 October 2016 Accepted: 21 March 2017 Published: 6 June 2017
Abstract
The response of underground plant tissues to O2 limitation is currently an important topic in crop plants since adverse environmental conditions (e.g. waterlogging) may cause root hypoxia and thus compromise plant growth. However, little is known on the effect of low O2 conditions in leaves, probably because O2 limitation is improbable in these tissues under natural conditions, unless under complete submersion. Nevertheless, an O2-depleted atmosphere is commonly used in gas exchange experiments to suppress photorespiration and estimate gross photosynthesis. However, the nonphotosynthetic effects of gaseous O2 depletion, particularly on respiratory metabolism, are not well documented. Here, we used metabolomics obtained under contrasting O2 and CO2 conditions to examine the specific effect of a changing O2 mole fraction from ambient (21%) to 0%, 2% or 100%. In addition to the typical decrease in photorespiratory intermediates (glycolate, glycine and serine) and a build-up in photosynthates (sucrose), low O2 (0% or 2%) was found to trigger an accumulation of alanine and change succinate metabolism. In 100% O2, the synthesis of threonine and methionine from aspartate appeared to be stimulated. These responses were observed in two species, sunflower (Helianthus annuus L.) and Arabidopsis thaliana (L.) Heynh. Our results show that O2 causes a change in the oxygenation : carboxylation ratio and also alters postphotosynthetic metabolism: (i) a hypoxic response at low O2 mole fractions and (ii) a stimulation of S metabolism at high O2 mole fractions. The latter effect is an important piece of information to better understand how photorespiration may control S assimilation.
Additional keywords: methionine, mitochondrial respiration, oxygen deficiency, photosynthesis.
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