Use of infrared thermography for monitoring crassulacean acid metabolism
Bronwyn J. Barkla A B and Timothy Rhodes A
+ Author Affiliations
- Author Affiliations
A Southern Cross Plant Science, Southern Cross University, Military Road, Lismore, NSW 2480, Australia.
B Corresponding author. Email: bronwyn.barkla@scu.edu.au
Functional Plant Biology 44(1) 46-51 https://doi.org/10.1071/FP16210
Submitted: 9 June 2016 Accepted: 31 August 2016 Published: 5 October 2016
Abstract
Crassulacean acid metabolism (CAM) is an alternative carbon fixation pathway that imparts high water-use efficiency in plants adapted to warm, semiarid climates. With concerns that global warming will negatively influence crop production, turning agricultural focus towards CAM plants may provide a solution to increase productivity using either unconventional crops on marginal land or incorporating CAM molecular mechanisms into conventional crops and improving water-use efficiency. For this to be feasible, deeper insights into CAM pathway regulation are essential. To facilitate this research new tools which simplify procedures for detecting and measuring CAM are needed. Here we describe a non-invasive, non-destructive, simplified method using infrared thermography for monitoring CAM in the annual desert succulent Mesembryanthemum crystallinum L. via detection of changes in leaf temperature brought about by the absence of transpiration due to daytime reduction in stomatal conductance. This method is sensitive, measuring temperature differences of ± 1°C, can be used in both the field and green house and is not restricted by leaf architecture. It offers an alternative to the commonly used gas exchange methods to measure CAM that are technically difficult to acquire and require the use of expensive and cumbersome equipment.
Additional keywords: ice plant, imaging, leaf temperature, gas exchange, salinity, transpiration.
References
Adams P, Nelson DE, Yamada S, Chmara W, Jensen RG, Bohnert HJ, Griffiths H (1998) Growth and development of Mesembryanthemum crystallinum (Aizoaceae). New Phytologist 138, 171–190.| Growth and development of Mesembryanthemum crystallinum (Aizoaceae).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXitlOhurs%3D&md5=44bc1e2efa2f2155e625f40d3d185ddcCAS |
Borland A, Wullschleger SD, Weston DJ, Hartwell J, Tuskan GA, Yang X, Cushman JC (2015) Climate-resilient agroforestry: physiological responses to climate change and engineering of crassulacean acid metabolism (CAM) as a mitigating strategy. Plant, Cell & Environment 38, 1833–1849.
| Climate-resilient agroforestry: physiological responses to climate change and engineering of crassulacean acid metabolism (CAM) as a mitigating strategy.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXht1yqsLnP&md5=a2cfae96e0b3b533abeea721b101c925CAS |
Costa JM, Grant OM, Chaves MM (2013) Thermography to explore plant-environment interactions. Journal of Experimental Botany 64, 3937–3949.
| Thermography to explore plant-environment interactions.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhs1SmtLbK&md5=729780e5ddbbf46c5ff3a3402f75b9aeCAS | 23599272PubMed |
Curtis OF (1936) Transpiration and cooling of leaves. American Journal of Botany 23, 7–10.
| Transpiration and cooling of leaves.Crossref | GoogleScholarGoogle Scholar |
Cushman JC, Tillet RL, Wood JA, Branco JM, Schlauch KA (2008) Large-scale mRNA expression profiling in the common ice plant, Mesembryanthemum crystallinum, performing C3 photosynthesis and Crassulacean acid metabolism (CAM). Journal of Experimental Botany 59, 1875–1894.
| Large-scale mRNA expression profiling in the common ice plant, Mesembryanthemum crystallinum, performing C3 photosynthesis and Crassulacean acid metabolism (CAM).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXmtlelsb0%3D&md5=258e558c0fc54c25c078988813ba55b9CAS | 18319238PubMed |
Defraeye T, Verboven P, Ho QT, Nicolai B (2013) Convective heat and mass exchange predictions at leaf surfaces: applications, methods and perspectives. Computers and Electronics in Agriculture 96, 180–201.
| Convective heat and mass exchange predictions at leaf surfaces: applications, methods and perspectives.Crossref | GoogleScholarGoogle Scholar |
DePaoli HC, Borland AM, Tuskan GA, Cushman JC, Yang X (2014) Synthetic biology as it relates to CAM photosynthesis: challenges and opportunities. Journal of Experimental Botany 65, 3381–3393.
| Synthetic biology as it relates to CAM photosynthesis: challenges and opportunities.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXhtFCrurnN&md5=ef5c03dab1d67e33ed874a40008f9862CAS | 24567493PubMed |
Gross SM, Martin JA, Simpson J, Abraham-Juarez MJ, Wang Z, Visel A (2013) De novo transcriptome assembly of drought tolerant CAM plants, Agave deserti and Agave tequilana. BMC Genomics 14, 563
| De novo transcriptome assembly of drought tolerant CAM plants, Agave deserti and Agave tequilana.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhsVKrt7nP&md5=3632bb4f04b6a78d454d509b7a68bc98CAS | 23957668PubMed |
Kaplan H (2007) ‘Practical applications of infrared thermal sensing and imaging equipment.’ (3rd edn) (SPIE Press: Washington DC)
Luo R, Wei H, Ye L, Wang K, Chen F, Luo L, Liu L, Li Y, Crabbe MJC, Jin L, Li Y, Zhong Y (2009) Photosynthetic metabolism of C3 plants shows highly cooperative regulation under changing environments: a systems biological analysis. Proceedings of the National Academy of Sciences of the United States of America 106, 847–852.
| Photosynthetic metabolism of C3 plants shows highly cooperative regulation under changing environments: a systems biological analysis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXht12isbk%3D&md5=ec7b0da9d2440297bc6647510ea668c4CAS | 19129487PubMed |
Lüttge U (2004) Ecophysiology of Crassulacean Acid Metabolism (CAM). Annals of Botany 93, 629–652.
| Ecophysiology of Crassulacean Acid Metabolism (CAM).Crossref | GoogleScholarGoogle Scholar | 15150072PubMed |
Ming R, VanBuren R, Wai CM, Tang H, Schatz MC, Bowers JE, Lyons E, Wang ML, Chen J, Biggers E, et al (2015) The pineapple genome and the evolution of CAM photosynthesis. Nature Genetics 47, 1435–1442.
| The pineapple genome and the evolution of CAM photosynthesis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXhslKgt7nN&md5=89f901660b5fe7efc549eb22ba2fc7e7CAS | 26523774PubMed |
Oh D-H, Barkla BJ, Vera‐Estrella R, Pantoja O, Lee S-Y, Bohnert HJ, Dassanayake M (2015) Cell type-specific responses to salinity – the epidermal bladder cell transcriptome of Mesembryanthemum crystallinum. New Phytologist 207, 627–644.
| Cell type-specific responses to salinity – the epidermal bladder cell transcriptome of Mesembryanthemum crystallinum.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXhtFOjs7bN&md5=486f5866ac3f2afcf34469225bac3731CAS | 25944243PubMed |
Osmond CB (1978) Crassulacean acid metabolism: a curiosity in context. Annual Review of Plant Physiology 29, 379–414.
| Crassulacean acid metabolism: a curiosity in context.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE1cXktl2gt7c%3D&md5=e1c32a890e432c2bd076ed91cc5a8a3fCAS |
Prytz G, Futsaether CM, Johnsson A (2003) Thermography studies of the spatial and temporal variability in stomatal conductance of Avena leaves during stable and oscillatory transpiration. New Phytologist 158, 249–258.
| Thermography studies of the spatial and temporal variability in stomatal conductance of Avena leaves during stable and oscillatory transpiration.Crossref | GoogleScholarGoogle Scholar |
Sage RF (2014) Photosynthetic efficiency and carbon concentration in terrestrial plants: the C4 and CAM solutions. Journal of Experimental Botany 65, 3323–3325.
| Photosynthetic efficiency and carbon concentration in terrestrial plants: the C4 and CAM solutions.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXhtFCrurnM&md5=61b509143d8f66af62424d0e65434bccCAS | 25136710PubMed |
Vera-Estrella R, Barkla BJ, Amezcua-Romero JC, Pantoja O (2012) Day/night regulation of aquaporins during the cam cycle in Mesembryanthemum crystallinum. Plant, Cell & Environment 35, 485–501.
| Day/night regulation of aquaporins during the cam cycle in Mesembryanthemum crystallinum.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XktV2gurY%3D&md5=541626fc35ccfb93a435eaf8b22a95b2CAS |
Walter H, Stadelmann E (1974) A new approach to the water relations of desert plants. In ‘Desert biology: special topics on the physical and biological aspects of arid regions’. (Ed. GW Brown) pp. 214–302. (Academic Press: London)
Winter K, Smith JAC (1996) Crassulacean acid metabolism: current status and perspectives. In ‘Crassulacean acid metabolism: biochemistry, ecophysiology and evolution’. (Eds K Winter, JAC Smith) pp. 389–420. (Springer: New York)
Winter K, Ziegler H (1992) Induction of Crassulacean acid metabolism in Mesembryanthemum crystallinum increases reproductive success under conditions of drought and salinity stress. Oecologia 92, 475–479.
| Induction of Crassulacean acid metabolism in Mesembryanthemum crystallinum increases reproductive success under conditions of drought and salinity stress.Crossref | GoogleScholarGoogle Scholar |
Winter K, Aranda J, Holtum JAM (2005) Carbon isotope composition and water-use efficiency in plants with Crassulacean acid metabolism. Functional Plant Biology 32, 381–388.
| Carbon isotope composition and water-use efficiency in plants with Crassulacean acid metabolism.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXks1ehsr0%3D&md5=a010b2e3bbea568c14b0b6aea52a4b09CAS |
Winter K, Holtum JAM (2014) Facultative crassulacean acid metabolism (CAM) plants: powerful tools for unravelling the functional elements of CAM photosynthesis. Journal of Experimental Botany 65, 3425–3441.
| Facultative crassulacean acid metabolism (CAM) plants: powerful tools for unravelling the functional elements of CAM photosynthesis.Crossref | GoogleScholarGoogle Scholar | 24642847PubMed |
Yang X, Cushman JC, Borland AM, Edwards EJ, Wullschleger SD, Tuskan GA, Owen NA, Griffiths H, Smith JA, De Paoli HC, et al (2015) A roadmap for research on Crassulacean acid metabolism (CAM) to enhance sustainable food and bioenergy production in a hotter, drier world. New Phytologist 207, 491–504.
| A roadmap for research on Crassulacean acid metabolism (CAM) to enhance sustainable food and bioenergy production in a hotter, drier world.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXhtFOjs7bK&md5=02810f6549be2d76676d0d2a0b055214CAS | 26153373PubMed |
