Natural variation in primary root growth and K+ retention in roots of habanero pepper (Capsicum chinense) under salt stress
Emanuel Bojórquez-Quintal A C , Nancy Ruiz-Lau A D , Ana Velarde-Buendía B , Ileana Echevarría-Machado A , Igor Pottosin B and Manuel Martínez-Estévez A E
+ Author Affiliations
- Author Affiliations
A Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Yucatán, México.
B Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colima, México.
C CONACYT-Laboratorio de Análisis y Diagnóstico del Patrimonio, El Colegio de Michoacán, La Piedad, Michoacán.
D CONACYT-Instituto Tecnológico Nacional de México, Instituto Tecnológico de Tuxtla Gutiérrez, Tuxtla Gutiérrez, México.
E Corresponding author. Email: luismanh@cicy.mx
Functional Plant Biology 43(12) 1114-1125 https://doi.org/10.1071/FP15391
Submitted: 26 December 2015 Accepted: 24 July 2016 Published: 19 August 2016
Abstract
In this work, we analysed the natural variation in mechanisms for protection against salt stress in pepper varieties (Capsicum chinense Jacq. cv. Rex, Chichen-Itza and Naranja and Capsicum annuum L. cv. Padron), considering primary root growth and viability of the post-stressed seedlings. NaCl-induced K+ and H+ efflux in roots was also studied by ion-selective microelectrodes under application of pharmacological agents. In these pepper varieties, the magnitude of the K+ leakage in the roots positively correlated with growth inhibition of the primary root in the presence of NaCl, with Rex variety showing a higher level of tolerance than Chichen-Itza. The K+ leakage and the activity of the H+ pump in the roots were dependent on the NaCl concentration. Pharmacological analysis indicated that the NaCl-induced K+ leakage was mediated by TEA+-sensitive KOR channels but not by NSCC channels. In addition, we present evidence for the possible participation of proline, and a Na+-insensitive HAK K+ transporter expressed in habanero pepper roots for maintaining K+ homeostasis under salt stress conditions.
Additional keywords: Capsicum annuum, Capsicum chinense, ion-selective electrodes, K+ retention, K+ efflux, primary root.
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