Expression and functional analysis of PhEOL1 and PhEOL2 during flower senescence in petunia
Juanxu Liu A B , Ji Zhao A , Zhina Xiao A , Xinlei Chang A , Guoju Chen B and Yixun Yu A B C
+ Author Affiliations
- Author Affiliations
A Guangdong Key Laboratory for Innovative Development and Utilisation of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China.
B College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
C Corresponding author. Email: yuyixun@scau.edu.cn
Functional Plant Biology 43(5) 413-422 https://doi.org/10.1071/FP15311
Submitted: 30 September 2015 Accepted: 8 January 2016 Published: 29 February 2016
Abstract
The ethylene biosynthesis pathway controls flower senescence. Previous studies have shown that Arabidopsis ETHYLENE-OVERPRODUCER1 (ETO1) interacts specifically with and negatively regulates type 2 1-aminocyclopropane-1-carboxylate synthases (ACSs), the rate-limiting enzymes of ethylene biosynthesis. The ethylene biosynthesis pathway controls flower senescence in petunias (Petunia hybrida Juss.). However, the role of ETO1-like genes (EOLs) during flower senescence has not been investigated. Here, two full-length petunia EOL cDNAs, PhEOL1 and PhEOL2, were isolated. RT–PCR assays indicated that the expression of PhEOL1 and PhEOL2 increased after exogenous ethylene treatment. The VIGS-mediated silencing of PhEOL1 accelerated flower senescence and produced more ethylene than the control condition, whereas the silencing of PhEOL2 did not. Notably, the effects caused by PhEOL1 suppression were not enhanced by PhEOL2 suppression in corollas. In addition, the expression of two petunia type 2 PhACS genes increased during flower senescence and after ethylene treatment. A yeast two-hybrid assay showed that PhEOL1 interacts with both PhACS2 and PhACS3. It is possible that PhEOL1 is involved in flower senescence by interacting with type 2 PhACSs in petunias.
Additional keywords: ACC synthase, ethylene, ETO1-like, Petunia, VIGS.
References
Abeles FB, Morgan PW, Saltveit ME, Jr (1992) ‘Ethylene in plant biology. (Academic Press: San Diego, CA, USA)Argueso CT, Hansen M, Kieber JJ (2007) Regulation of ethylene biosynthesis. Journal of Plant Growth Regulation 26, 92–105.
| Regulation of ethylene biosynthesis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXot1yks7o%3D&md5=150a058bf86a26a92be710b663573930CAS |
Benavente LM, Alonso JM (2006) Molecular mechanisms of ethylene signaling in Arabidopsis. Molecular BioSystems 2, 165–173.
| Molecular mechanisms of ethylene signaling in Arabidopsis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XjvFOjsbk%3D&md5=52bd1bbeda88f949477e4ea28726d102CAS | 16880934PubMed |
Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Wittwer CT (2009) The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clinical Chemistry 55, 611–622.
| The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXktVWqs7g%3D&md5=354c9eabd72fa1987819b037e556239fCAS | 19246619PubMed |
Christians MJ, Gingerich DJ, Hansen M, Binder BM, Kieber JJ, Vierstra RD (2009) The BTB ubiquitin ligases ETO1, EOL1 and EOL2 act collectively to regulate ethylene biosynthesis in Arabidopsis by controlling type‐2 ACC synthase levels. The Plant Journal 57, 332–345.
| The BTB ubiquitin ligases ETO1, EOL1 and EOL2 act collectively to regulate ethylene biosynthesis in Arabidopsis by controlling type‐2 ACC synthase levels.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhs1Sgtbw%3D&md5=b99d3b919912c2062126c6f8f26e7f03CAS | 18808454PubMed |
Force A, Lynch M, Pickett FB, Amores A, Yan Y, Postlethwait J (1999) Preservation of duplicate genes by complementary, degenerative mutations. Genetics 151, 1531–1545.
Fu Z, Wang H, Liu J, Liu J, Wang J, Zhang Z, Yu Y (2011) Cloning and characterization of a DCEIN2 gene responsive to ethylene and sucrose in carnation cut flower. Plant Cell, Tissue and Organ Culture 105, 447–455.
| Cloning and characterization of a DCEIN2 gene responsive to ethylene and sucrose in carnation cut flower.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXmsVyjt7w%3D&md5=1c6092940374be458dfc2a1c35fcedd1CAS |
Guzman P, Ecker JR (1990) Exploiting the triple response of Arabidopsis to identify ethylene-related mutants. The Plant Cell 2, 513–523.
| Exploiting the triple response of Arabidopsis to identify ethylene-related mutants.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3cXlsV2jsbw%3D&md5=4aa6ba9d73278ac425b74d6e0362e404CAS | 2152173PubMed |
Huang L, Lai U, Yang S, Chu M, Kuo C, Tsai M, Sun C (2007) Delayed flower senescence of Petunia hybrida plants transformed with antisense broccoli ACC synthase and ACC oxidase genes. Postharvest Biology and Technology 46, 47–53.
| Delayed flower senescence of Petunia hybrida plants transformed with antisense broccoli ACC synthase and ACC oxidase genes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXhtVSjs7jL&md5=9bcfd7a1fdd077c204141327b19b97e7CAS |
Ji Y, Guo H (2013) From endoplasmic reticulum (ER) to nucleus: EIN2 bridges the gap in ethylene signaling. Molecular Plant 6, 11–14.
| From endoplasmic reticulum (ER) to nucleus: EIN2 bridges the gap in ethylene signaling.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhtFCnurw%3D&md5=e9cc6d72f427fb7ed69df7e61df76815CAS | 23239828PubMed |
Kende H (1993) Ethylene biosynthesis. Annual Review of Plant Biology 44, 283–307.
| Ethylene biosynthesis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXlsFKisbo%3D&md5=64e6d9c6845601fe0092a691e28c4e4aCAS |
Kieber JJ, Rothenberg M, Roman G, Feldmann KA, Ecker JR (1993) CTR1, a negative regulator of the ethylene response pathway in Arabidopsis, encodes a member of the raf family of protein kinases. Cell 72, 427–441.
| CTR1, a negative regulator of the ethylene response pathway in Arabidopsis, encodes a member of the raf family of protein kinases.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXit1Ojsbg%3D&md5=5585553a16ae60c4f0593858ab1d8a88CAS | 8431946PubMed |
Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Briefings in Bioinformatics 5, 150–163.
| MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXntFGqu7s%3D&md5=6c92581e54f2e5d4f9e484398ede8963CAS | 15260895PubMed |
Liang X, Shen NF, Theologis A (1996) Li+‐regulated 1‐aminocyclopropane‐1‐carboxylate synthase gene expression in Arabidopsis thaliana. The Plant Journal 10, 1027–1036.
| Li+‐regulated 1‐aminocyclopropane‐1‐carboxylate synthase gene expression in Arabidopsis thaliana.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXnsV2ksA%3D%3D&md5=cfd7b067ecf9ab0d63164459674514ffCAS | 9011084PubMed |
Liu J, Li J, Wang H, Fu Z, Liu J, Yu Y (2011) Identification and expression analysis of ERF transcription factor genes in petunia during flower senescence and in response to hormone treatments. Journal of Experimental Botany 62, 825–840.
| Identification and expression analysis of ERF transcription factor genes in petunia during flower senescence and in response to hormone treatments.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhsFyrsb%2FF&md5=1886d628112e49a3650215772e4f7a8cCAS | 20974735PubMed |
Mallona I, Lischewski S, Weiss J, Hause B, Egea-Cortines M (2010) Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida. BMC Plant Biology 10, 4
| Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida.Crossref | GoogleScholarGoogle Scholar | 20056000PubMed |
Sato T, Theologis A (1989) Cloning the mRNA encoding 1-aminocyclopropane-1-carboxylate synthase, the key enzyme for ethylene biosynthesis in plants. Proceedings of the National Academy of Sciences of the United States of America 86, 6621–6625.
| Cloning the mRNA encoding 1-aminocyclopropane-1-carboxylate synthase, the key enzyme for ethylene biosynthesis in plants.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL1MXlvVylsbc%3D&md5=aa35c3cb7e5ea9fab356013c3c1c427fCAS | 2671999PubMed |
Spanu P, Felix G, Boller T (1990) Inactivation of stress induced 1-aminocyclopropane carboxylate synthase in vivo differs from substrate-dependent inactivation in vitro. Plant Physiology 93, 1482–1485.
| Inactivation of stress induced 1-aminocyclopropane carboxylate synthase in vivo differs from substrate-dependent inactivation in vitro.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3cXmtVyntrs%3D&md5=6586b81296f86a8574ea8acd587de7a3CAS | 16667643PubMed |
Spanu P, Grosskopf DG, Felix G, Boller T (1994) The apparent turnover of 1-aminocyclopropane-1-carboxylate synthase in tomato cells is regulated by protein phosphorylation and dephosphorylation. Plant Physiology 106, 529–535.
Spitzer-Rimon B, Farhi M, Albo B, Cna Ani A, Zvi MMB, Masci T, Edelbaum O, Yu Y, Shklarman E, Ovadis M (2012) The R2R3-MYB–like regulatory factor EOBI, acting downstream of EOBII, regulates scent production by activating ODO1 and structural scent-related genes in Petunia. The Plant Cell 24, 5089–5105.
| The R2R3-MYB–like regulatory factor EOBI, acting downstream of EOBII, regulates scent production by activating ODO1 and structural scent-related genes in Petunia.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXisVKktL0%3D&md5=9c44e3d24841652c2cfbe01e8767c819CAS | 23275577PubMed |
Tan Y, Liu J, Huang F, Guan J, Zhong S, Tang N, Zhao J, Yang W, Yu Y (2014) PhGRL2 protein, interacting with PhACO1, is involved in flower senescence in the petunia. Molecular Plant 7, 1384–1387.
| PhGRL2 protein, interacting with PhACO1, is involved in flower senescence in the petunia.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXktlKltrk%3D&md5=ebfd81aa8b2681274b7c3b4779141d2bCAS | 24618881PubMed |
Wang KL, Li H, Ecker JR (2002) Ethylene biosynthesis and signaling networks. The Plant Cell 14, S131–S151.
Wang KL, Yoshida H, Lurin C, Ecker JR (2004) Regulation of ethylene gas biosynthesis by the Arabidopsis ETO1 protein. Nature 428, 945–950.
| Regulation of ethylene gas biosynthesis by the Arabidopsis ETO1 protein.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXjsV2mtro%3D&md5=bb94784d720a01bb272b51bf279b2697CAS | 15118728PubMed |
Woeste KE, Ye C, Kieber JJ (1999) Two Arabidopsis mutants that overproduce ethylene are affected in the posttranscriptional regulation of 1-aminocyclopropane-1-carboxylic acid synthase. Plant Physiology 119, 521–530.
| Two Arabidopsis mutants that overproduce ethylene are affected in the posttranscriptional regulation of 1-aminocyclopropane-1-carboxylic acid synthase.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXhsFSksr8%3D&md5=ed771675ba7ffca0243b1e137404b496CAS | 9952448PubMed |
Yoshida H, Nagata M, Saito K, Wang KL, Ecker JR (2005) Arabidopsis ETO1 specifically interacts with and negatively regulates type 2 1-aminocyclopropane-1-carboxylate synthases. BMC Plant Biology 5, 14
| Arabidopsis ETO1 specifically interacts with and negatively regulates type 2 1-aminocyclopropane-1-carboxylate synthases.Crossref | GoogleScholarGoogle Scholar | 16091151PubMed |
Yoshida H, Wang KL, Chang C, Mori K, Uchida E, Ecker JR (2006) The ACC synthase TOE sequence is required for interaction with ETO1 family proteins and destabilization of target proteins. Plant Molecular Biology 62, 427–437.
| The ACC synthase TOE sequence is required for interaction with ETO1 family proteins and destabilization of target proteins.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XhtVSitLfK&md5=317d20bd4c0c8a535b56fc1904769330CAS | 16897471PubMed |
Yu Y, Wang H, Liu J, Fu Z, Wang J (2011) Transcriptional regulation of two RTE-like genes of carnation during flower senescence and upon ethylene exposure, wounding treatment and sucrose supply. Plant Biology 13, 719–724.
| Transcriptional regulation of two RTE-like genes of carnation during flower senescence and upon ethylene exposure, wounding treatment and sucrose supply.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhtFCjtL7O&md5=be1756ba70b1ac3cf8372b50cfad30e9CAS | 21815975PubMed |
