Hydrogen peroxide promotes programmed cell death and salicylic acid accumulation during the induced production of sesquiterpenes in cultured cell suspensions of Aquilaria sinensis
Juan Liu A , Yanhong Xu A , Zheng Zhang A B and Jianhe Wei A B C
+ Author Affiliations
- Author Affiliations
A Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 151, Malianwa North Road, Haidian District, Beijing 100193, China.
B Hainan Branch Institute of Medicinal Plant Development (Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine), Chinese Academy of Medical Sciences & Peking Union Medical College, Wanning 571533, China.
C Corresponding author. Email: weijianhe126@126.com; wjianh@263.net
Functional Plant Biology 42(4) 337-346 https://doi.org/10.1071/FP14189
Submitted: 20 July 2014 Accepted: 7 November 2014 Published: 2 December 2014
Abstract
Aquilaria sinensis (Lour.) Gilg produces a highly valuable agarwood characterised by a diverse array of sesquiterpenes and chromone derivatives that can protect wounded trees against potential herbivores and pathogens. A defensive reaction on the part of the plant has been proposed as the key reason for agarwood formation, but the issue of whether programmed cell death (PCD), an important process of plant immune responding, is involved in agarwood formation, still needs to be clarified. In this study, treatment of cultured cell suspensions with hydrogen peroxide (H2O2) induced the production of sesquiterpenes due to endogenous accumulation of salicylic acid (SA) and elevations in the expression of sesquiterpene biosynthetic genes. Moreover, PCD was stimulated by H2O2 in cultured cell suspensions of A. sinensis due to the induction of caspase activity, upregulated expression of metacaspases and cytochrome c, and SA accumulation. Our findings demonstrate for the first time that H2O2 stimulates PCD, SA accumulation and sesquiterpene production in cultured cell suspensions of A. sinensis. Furthermore, results from this study provide a valuable insight into investigations of the potential interactions between sesquiterpene synthesis and PCD during agarwood formation.
Additional keywords: agarwood, PCD, reactive oxygen species, ROS, SA, salicylic acid.
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