Transcriptional regulation of phosphate transporters from Lolium perenne and its mycorrhizal symbionts in response to phosphorus supply
Qianhe Liu A , Anthony J. Parsons B , Hong Xue A , Chris S. Jones A and Susanne Rasmussen A B C
+ Author Affiliations
- Author Affiliations
A AgResearch, Tennent Drive, PB 11 008, Palmerston North 4442, New Zealand.
B Present address: Institute of Agriculture and Environment, Massey University, Palmerston North 4474, New Zealand.
C Corresponding author. Email: s.rasmussen@massey.ac.nz
Functional Plant Biology 42(1) 1-8 https://doi.org/10.1071/FP14043
Submitted: 3 February 2014 Accepted: 25 June 2014 Published: 28 August 2014
Abstract
Phosphate (P) uptake is critical for plant growth, but to date little is known about P uptake and transport in the pasture grass Lolium perenne L. We have identified a putative P transporter (PT) from L. perenne mycorrhizal roots (LpPT1) and assessed its transcriptional regulation by soil P availability and mycorrhizal colonisation. We also investigated transcript levels of fungal PTs from the two arbuscular mycorrhizal species Rhizophagus intraradices and Funneliformis mosseae. Our analyses indicated that LpPT1 codes for a high affinity PT most likely responsible for direct P uptake from the soil. LpPT1 is highly expressed in roots of plants grown at low P, whereas high P repressed its expression. LpPT1 was not expressed in above-ground plant tissues. Colonisation with R. intraradices did not affect expression of LpPT1 significantly. Transcript levels of the R. intraradices PT were not affected by P availability but the F. mosseae PT was repressed by high P supply, particularly in intraradical hyphae. Our study could assist in deciphering the molecular mechanisms of P uptake in the pasture grass L. perenne.
Additional keywords: Funneliformis (Glomus) mosseae, perennial ryegrass, Rhizophagus (Glomus) intraradices, transcript profiling.
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