Over-expression of SlCycA3 gene in Arabidopsis accelerated the cell cycle transition
Jia Guo A , Jiawen Wu A , Tiantian Zhang A and Haijun Gong A B
+ Author Affiliations
- Author Affiliations
A College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China.
B Corresponding author. Email: gongnavy@163.com
Functional Plant Biology 41(6) 659-670 https://doi.org/10.1071/FP13335
Submitted: 5 June 2013 Accepted: 18 December 2013 Published: 30 January 2014
Abstract
We characterised an A-type cyclin SlCycA3 (AJ243453) from tomato (Solanum lycopersicum L.). Phylogenetic analysis based on the deduced amino acid sequence revealed that SlCycA3 was 71% identical to A3-type cyclin in Nicotiana tabacum L. (CAA63540), 48% identical to its homologue found in Arabidopsis thaliana (NP_199122), and 48% identical to its homologue in Pisum sativum L. (CAB77269). SlCycA3 gene was transformed into Arabidopsis plants in order to study its function. The hypocotyl length of transgenic plants was approximately half the length of wild-type plants, and the cell size in the transgenic lines was also smaller. The transgenic plants had longer roots than the wild type. Overexpression of SlCycA3 gene accelerated the cell cycle from G1/S transition to early M-phase, thereby accelerating the cell division. When the plants were treated with IAA and 3-indolebutyric acid (IBA) for 2 days, the transgenic plants produced more lateral roots than wild type. Treatment with IBA significantly increased the cell number in the G2-phase in transgenic plants compared with wild type after treatment for 10 days, whereas the proportion of cells in the S-phase was strongly increased by IAA treatment both in wild-type and transgenic plants. These results suggest a possible key role for cyclin in regulating root growth and development and provide some evidence of cell division underlying hormone treatment in plants.
Additional keywords: Arabidopsis, cyclin, hypocotyl, SlCycA3, root growth.
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