Comparative performance of δ13C, δ18O and δ15N for phenotyping durum wheat adaptation to a dryland environment
José Luis Araus A D , Llorenç Cabrera-Bosquet A C , María Dolores Serret A , Jordi Bort A and María Teresa Nieto-Taladriz B
+ Author Affiliations
- Author Affiliations
A Unitat de Fisiologia Vegetal, Facultat de Biologia, Universitat de Barcelona, 08028 Barcelona, Spain.
B Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra. de La Coruña Km. 7,5, 28040 Madrid, Spain.
C Present address: INRA, UMR759 Laboratoire d’Ecophysiologie des Plantes sous Stress Environnementaux. Place Viala, F-34060 Montpellier, France.
D Corresponding author. Email: jaraus@ub.edu
Functional Plant Biology 40(6) 595-608 https://doi.org/10.1071/FP12254
Submitted: 31 August 2012 Accepted: 25 January 2013 Published: 7 March 2013
Abstract
Grain yield and the natural abundance of the stable isotope compositions of carbon (δ13C), oxygen (δ18O) and nitrogen (δ15N) of mature kernels were measured during 3 consecutive years in 10 durum wheat genotypes (five landraces and five modern cultivars) subjected to different water and N availabilities in a Mediterranean location and encompassing a total of 12 trials. Water limitation was the main environmental factor affecting yield, δ13C and δ18O, whereas N fertilisation had a major effect on δ15N. The genotypic effect was significant for yield, yield components, δ13C, δ18O and δ15N. Landraces exhibited a higher δ13C and δ15N than cultivars. Phenotypic correlations of δ13C and δ18O with grain yield were negative, suggesting that genotypes able to sustain a higher water use and stomatal conductance were the most productive and best adapted; δ15N was also negatively correlated with grain yield regardless of the growing conditions. δ13C was the best isotopic trait in terms of genetic correlation with yield and heritability, whereas δ18O was the worst of the three isotopic abundances. The physiological basis for the different performance of the three isotopes explaining the genotypic variability in yield is discussed.
Additional keywords: carbon, drought, nitrogen availability, oxygen and nitrogen stable isotopes, phenotyping, yield.
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